Polyacrylamide Gel Electrophoresis
Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R. Green, Joseph Sambrook
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Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters [PDF]
Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis. [PDF]
Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Magdeldin S +8 more
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A Viscosity Model of Polyacrylamide Gel Electrophoresis [PDF]
In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted “pores”) to different-sized ions due to the effect of “geometric exclusion”.
Hans-Joachim Bode
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis [PDF]
UE Loening
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Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE [PDF]
Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
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Analysis of Mitochondrial Respiratory Chain Supercomplexes Using Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE). [PDF]
Jha P, Wang X, Auwerx J.
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Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand +5 more
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Functional characterization of reductive dehalogenases by using blue native polyacrylamide gel electrophoresis. [PDF]
Tang S +7 more
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