Results 1 to 10 of about 513,630 (294)

The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]

open access: yesBiochemical Journal, 1980
The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
Evans, RW, Williams, J
core   +6 more sources

A Viscosity Model of Polyacrylamide Gel Electrophoresis [PDF]

open access: hybridZeitschrift für Naturforschung C, 1979
In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular siev­ing relies on different accessibility of volume fractions and of cross-sectional area fractions (denot­ed “pores”) to different-sized ions due to the effect of “geometric exclusion”.
Hans-Joachim Bode
openalex   +4 more sources

Denaturing Polyacrylamide Gel Electrophoresis [PDF]

open access: yesCurrent Protocols in Nucleic Acid Chemistry, 1994
AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
openaire   +4 more sources

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

open access: yes, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

open access: yes, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő   +3 more
core   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

open access: yes, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yes, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold   +225 more
core   +3 more sources

Silver Staining of Proteins in 2DE Gels [PDF]

open access: yes, 2009
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille   +3 more
core   +4 more sources

Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]

open access: yes, 1971
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
core   +4 more sources

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

open access: yes, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi   +103 more
core   +4 more sources

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