A versatile polyacrylamide gel electrophoresis based sulfotransferase assay [PDF]
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Polyacrylamide Gel Electrophoresis.
Cold Spring Harbor Protocols, 2020 Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation.
Michael R Green, J. Sambrook
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Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (
Albright, Lisa M., Slatko, Barton E.
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. [PDF]
Biochemical Journal, 1967 1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2.
U. Loening
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Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters [PDF]
BioTechniques, 2023 Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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Analysis of Mitochondrial Respiratory Chain Supercomplexes Using Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE). [PDF]
Curr Protoc Mouse Biol, 2016 Mitochondria are cellular organelles that harvest energy in the form of ATP through a process termed oxidative phosphorylation (OXPHOS), which occurs via the protein complexes of the electron transport chain (ETC).
Jha P, Wang X, Auwerx J.
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis. [PDF]
Clin Proteomics, 2014 Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Magdeldin S +8 more
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Functional characterization of reductive dehalogenases by using blue native polyacrylamide gel electrophoresis. [PDF]
Appl Environ Microbiol, 2013 Dehalococcoides mccartyi strains are obligate organohalide-respiring bacteria harboring multiple distinct reductive dehalogenase (RDase) genes within their genomes. A major challenge is to identify substrates for the enzymes encoded by these RDase genes.
Tang S +7 more
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Examination of the Protein Composition of the Cell Envelope of Escherichia coli by Polyacrylamide Gel Electrophoresis [PDF]
Journal of Bacteriology, 1970 An envelope preparation containing the cell wall and cytoplasmic membrane of Escherichia coli was obtained by breaking the cells with a French pressure cell and sedimentating the envelope fraction by ultracentrifugation.
C. Schnaitman
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A Viscosity Model of Polyacrylamide Gel Electrophoresis [PDF]
Zeitschrift für Naturforschung C, 1979 In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted “pores”) to different-sized ions due to the effect of “geometric exclusion”.
Hans-Joachim Bode
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