Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters [PDF]
BioTechniques, 2023 Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay [PDF]
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE [PDF]
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
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Advanced Biomedical Research, 2015 Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand +5 more
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Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]
Pesquisa Veterinária Brasileira, 2021 : Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo +4 more
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The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
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A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting [PDF]
ACS OmegaMarc R. Birtwistle +7 more
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A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels
BioTechniques, 2023 Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez +5 more
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Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
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Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling [PDF]
Archives of Biological Sciences, 2011 An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange ...
Asaduzzaman A.K.M. +2 more
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