Polyacrylamide Gel Electrophoresis.
Cold Spring Harbor Protocols, 2020 Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation.
Michael R Green, J. Sambrook
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Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters [PDF]
BioTechniques, 2023 Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. [PDF]
Biochemical Journal, 1967 1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2.
U. Loening
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis. [PDF]
Clin Proteomics, 2014 Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Magdeldin S +8 more
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A Viscosity Model of Polyacrylamide Gel Electrophoresis [PDF]
Zeitschrift für Naturforschung C, 1979 In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted “pores”) to different-sized ions due to the effect of “geometric exclusion”.
Hans-Joachim Bode
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay [PDF]
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE [PDF]
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
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Analysis of Mitochondrial Respiratory Chain Supercomplexes Using Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE). [PDF]
Curr Protoc Mouse Biol, 2016 Jha P, Wang X, Auwerx J.
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Advanced Biomedical Research, 2015 Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand +5 more
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Functional characterization of reductive dehalogenases by using blue native polyacrylamide gel electrophoresis. [PDF]
Appl Environ Microbiol, 2013 Tang S +7 more
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