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Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 1994 AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
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Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters [PDF]
BioTechniques, 2023 Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay [PDF]
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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A Viscosity Model of Polyacrylamide Gel Electrophoresis [PDF]
Zeitschrift für Naturforschung C, 1979 In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular sieving relies on different accessibility of volume fractions and of cross-sectional area fractions (denoted “pores”) to different-sized ions due to the effect of “geometric exclusion”.
Hans-Joachim Bode
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Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE [PDF]
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
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Advanced Biomedical Research, 2015 Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand +5 more
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The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
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Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]
Pesquisa Veterinária Brasileira, 2021 : Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo +4 more
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A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels
BioTechniques, 2023 Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez +5 more
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A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting [PDF]
ACS OmegaMarc R. Birtwistle +7 more
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