Results 121 to 130 of about 514,759 (312)

Production of Specific Fragments of {varphi}X174 Replicative Form DNA by a Restriction Enzyme from Haemophilus parainfluenzae, Endonuclease HP [PDF]

, 1973
A restriction endonuclease from Haemophilus parainfluenzae degrades {varphi}X174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic ...
Johnson, Paul H., Lee, Amy Shiu, Sinsheimer, Robert L.   +2 more
core  

Ultrasound‐Responsive Dual‐Prodrug Nanoassembly for “Fenestrae‐Restoration Strategy” in Liver Fibrosis Therapy

Advanced Materials, EarlyView.
ABSTRACT Liver fibrosis is a serious yet reversible intermediate stage in the progression of liver disease, which can ultimately advance to cirrhosis and hepatocellular carcinoma. Targeted and selective inhibition of activated hepatic stellate cells (aHSCs) has emerged as a promising therapeutic strategy for the treatment of liver fibrosis.
Shutong Liu, Mengyao Zhang, Zitong Qiu, Xinyi Liu, Yunsheng Dou, Sona Krizkova, Zheyi Li, Zbynek Heger, Nan Li   +8 more
wiley   +1 more source

Population diversity of sheep bot fly, Oestrus ovis (Linné, 1758) (Diptera: Oestridae: Oestrinae), using SDS polyacrylamide gel electrophoresis. [PDF]

Saudi J Biol Sci, 2022
Kamal M, Yasmeen G, Saher NU, Naz F, Rafiq N, Khan W, Iqbal MA, Kabir M, Mushtaq S, Hassan HU, Usman T.   +10 more
europepmc   +1 more source

RIG‐I Mediated Neuron‐Specific IFN Type 1 Signaling in FUS‐ALS Induces Neurodegeneration and Offers New Biomarker‐Driven Individualized Treatment Options for (FUS‐)ALS

Advanced Science, EarlyView.
Using iPSC‐derived motoneurons and postmortem tissue from FUS‐ALS patients, it is demonstrated that increased mitochondrial transcription leads to elevated cytosolic double‐stranded RNA (dsRNA) levels. This aberrant accumulation activates a RIG‐I–dependent innate immune response leading to neurodegeneration, which is amenable for FDA‐ and EMA‐approved ...
Marcel Naumann, Theresa M. Wierschin, Stefanie Kretschmer, Banaja P. Dash, Aaron Held, Andrea Salzinger, Kevin Peikert, Anže Karlek, Hannes Glaß, Dajana Großmann, René Günther, Susanne Petri, Annekathrin Rödiger, David Brenner, Francisco Pan‐Montojo, Eleonora Aronica, Markus Kipp, Vitaly Zimyanin, Jared Sterneckert, Torsten Grehl, Noah D. Seebacher, Tobias M. Böckers, Alberto Catanese, Brian J. Wainger, Patrick Oeckl, Min Ae Lee‐Kirsch, Andreas Hermann   +26 more
wiley   +1 more source

Chronic Hypoxia Disrupts Spermatogenesis Through ASXL2–EZH2–Mediated Microtubule Destabilization

Advanced Science, EarlyView.
This study reveals the mechanism by which chronic hypoxia impairs spermatogenesis via the ASXL2–EZH2 axis, hindering the transition of spermatids from round to elongated forms. Key findings reveal that under hypoxic conditions, downregulated ASXL2 expression reduces EZH2 binding to the CEP162 promoter, leading to decreased H3K27me3 modification and ...
Jun Yin, Mengjie Zhang, Wenying Liu, Wenlong Shen, Debao Li, Hongming Miao, Fang Deng, Gang Zhang, Yi Tian, Yi Zhang, Zhihu Zhao, Bing Ni   +11 more
wiley   +1 more source

The construction of a secretary eukaryotic expression plasmid of chondroitinase ABC Ⅰ

Chinese Journal of Contemporary Neurology and Neurosurgery, 2010
Objective To construct a secretary eukaryotic expression plasmid for chondroitinase ABCⅠ, and observe the expression of the target protein in a glioma cell line TJ905. Methods The DNA fragments encoding the signal peptide of basilar membrane 40 (BM40) as
Hui⁃ling GAO, Qian WANG, Shi⁃zhu YU, Xiu⁃ju CHEN, Cui⁃yun SUN, Tong⁃ling AN   +5 more
doaj  

Midbrain PAG Astrocytes Modulate Mouse Defensive and Panic‐Like Behaviors

Advanced Science, EarlyView.
Astrocytes in the midbrain periaqueductal gray (PAG) dynamically encode threat intensity and shape defensive action selection in mice. Real‐time Ca2+ imaging reveals robust astrocytic activation during predator odor and CO2 exposure. Aberrant astrocytic Ca2+ overactivation disrupts goal‐directed escape, biases behavior toward freezing, and induces ...
Ellane Barcelon, Kyungchul Noh, Minkyu Hwang, Yoon‐Jung Kim, Unjin Lee, Yeon Joo Ryu, Je‐Kyung Ryu, Sang Beom Jun, Se‐Young Choi, Woo‐Hyun Cho, Sung Joong Lee   +10 more
wiley   +1 more source

A Simpler and Faster Version of Two-Dimensional Gel Electrophoresis Using Vertical, Mini SDS-PAGE Apparatus [PDF]

Iranian Journal of Chemistry & Chemical Engineering, 2009
We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods.
Yaser Dorri, Biji T. Kurien, R. Hal Scofield   +2 more
doaj  

Phosphorylation of chloroplast ribulose bisphosphate carboxylase/oxygenase small subunit by an envelope-bound protein kinase in situ [PDF]

, 1983
A new protein kinase of the cAMP independent type was found to be bound to the outer envelope membrane of spinach chloroplasts. While stimulated by Mg2+ and inhibited by ADP, the enzyme showed no response to conventional protein substrates and was ...
Buchanan, Bob B., Soll, Jürgen
core  

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source