Results 101 to 110 of about 920,072 (304)

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Transverse urea gradient polyacrylamide gel electrophoresis of proteins.

, 2019
Both rCyp and rCyp-CsA were separately analyzed by the Transverse urea gradient polyacrylamide gel electrophoresis as described [51, 58].
Subrata Sau (77536), Soumitra Polley (605913), Soham Seal (6528518)   +2 more
core   +1 more source

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Kastenholz, B., David E. Garfin, Bernd Kastenholz, Garfin, D.E.   +3 more
core   +1 more source

Urinary cholesterol: its association with a macromolecular protein- lipid complex [PDF]

, 1984
The cholesterol-containing complexes in the urine of normal subjects and patients with diseases accompanied by hyperexcretion of urinary cholesterol were characterized. In normal subjects, the major portion of the recovered urinary cholesterol was eluted
Jüngst, Dieter, Siess, E., Weiser, H., Karl, Hans Josef   +3 more
core   +1 more source

A Viscosity Model of Polyacrylamide Gel Electrophoresis

Zeitschrift für Naturforschung C, 1979
In current theories of polyacrylamide gel electrophoresis, the idea prevails that molecular siev­ing relies on different accessibility of volume fractions and of cross-sectional area fractions (denot­ed “pores”) to different-sized ions due to the effect of “geometric exclusion”.
openaire   +2 more sources

Genetically Programmed Shape‐Morphing of Engineered Living Materials

Advanced Functional Materials, EarlyView.
Here, bacterial and mammalian engineered living materials (ELMs) are presented, in which living cells direct genetically programmed and enzymatically mediated shape‐morphing. The resulting deformation and shape‐recovery can be actuated in a genetically controlled manner by an ELM incorporating out‐of‐equilibrium counteracting biochemical reactions with
Jan Becker, Yuchen Liu, Miguel Baños, Rosanne Schmachtenberg, Mahmudul Hasan, Claudia Fink‐Straube, Luai R. Khoury, Wilfried Weber   +7 more
wiley   +1 more source

Genetic characterization of Mycobacterium avium isolates recovered from humans and animals in Australia [PDF]

, 1996
Genetic relationships amongst 115 mainly Australian isolates of Mycobacterium avium were assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29 ...
Gilbert, G.L., Feizabadi, M.M., Dawson, D., Chew, W., Robertson, I.D., Cousins, D.V., Hampson, D.J.   +6 more
core  

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Biological Procedures Online, 2009
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Mandal Nakul, Heegaard Steffen, Prause Jan, Honoré Bent, Vorum Henrik   +4 more
doaj   +1 more source

Protein‐Nanoarchitectures for Low‐Field Intracellular Electro‐Immunomodulation

Advanced Functional Materials, EarlyView.
DHQ‐SDH‐mediated binding of AuNPs drives the formation of interconnected, chain‐like nanoarchitectures with enhanced electrical conductivity. These conductive assemblies undergo macrophage uptake and enable low‐field electrical stimulation, promoting M1‐to‐M2 phenotypic switching with decreased iNOS and increased CD206 expression. Such protein‐mediated
Viktoriya Pakharenko, Djanira Rodrigues Negrão, Tanmay Gupta, Robert Jeng, Otavio Augusto Titton Dias, Maia Jaffer‐Diaz, Dinesh Christendat, Mohini Sain   +7 more
wiley   +1 more source

Two-Dimensional Blue Native/SDS Polyacrylamide Gel Electrophoresis for Analysis of Brazilian Bothrops Snake Venoms. [PDF]

Toxins (Basel), 2022
Ferreira de Oliveira N, Sachetto ATA, Santoro ML.   +2 more
europepmc   +1 more source