Proteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk. [PDF]
Aslebagh R +6 more
europepmc +1 more source
Fully recombinant protein‐based biomaterials execute complex Boolean logic for user‐programmable material degradation and concomitant therapeutic cargo release. Biologics, such as growth factors, can be incorporated within the crosslinkers as “drugamers”, while encapsulated cells can be released according to nested YES/OR/AND‐type logical operations ...
Murial L. Ross +3 more
wiley +1 more source
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining. [PDF]
LaRiviere WB +5 more
europepmc +1 more source
In the recondite pulmonary neoplastic milieu, DESMIN+ cancer‐associated fibroblasts function as the paramount stromal source of IL‐8, precipitating a deleterious immunological metamorphosis by engaging CXCR2 receptors on the myeloid lineage. This paracrine stimulus triggers a mechanotransduction cascade characterized by RhoA‐dependent actin ...
Xuyu Gu +7 more
wiley +1 more source
Two-Dimensional Polyacrylamide Gel Electrophoresis Coupled with Nanoliquid Chromatography-Tandem Mass Spectrometry-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in the MCF7 Breast Cancer Cell Line Transfected for Jumping Translocation Breakpoint Protein Overexpression. [PDF]
Jayathirtha M +5 more
europepmc +1 more source
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu +12 more
wiley +1 more source
Population diversity of sheep bot fly, Oestrus ovis (Linné, 1758) (Diptera: Oestridae: Oestrinae), using SDS polyacrylamide gel electrophoresis. [PDF]
Kamal M +10 more
europepmc +1 more source
Polyacrylamide Gel Electrophoresis
Yoshimasa Kawaguchi, Yu Mikame
openaire +1 more source
A time‐multiplexed nanobody‐functionalized organic electrochemical transistor enables rapid and sensitive detection of three respiratory viral proteins from saliva. Sequentially addressed multi‐gate electrodes on a shared channel achieve around 1 fm detection within 15 min without sample preprocessing. Clinical validation demonstrates high specificity,
Tianrui Chang +13 more
wiley +1 more source
The construction of a secretary eukaryotic expression plasmid of chondroitinase ABC Ⅰ
Objective To construct a secretary eukaryotic expression plasmid for chondroitinase ABCⅠ, and observe the expression of the target protein in a glioma cell line TJ905. Methods The DNA fragments encoding the signal peptide of basilar membrane 40 (BM40) as
Hui⁃ling GAO +5 more
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