Results 71 to 80 of about 920,072 (304)

Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae [PDF]

open access: yes, 1989
Lipopolysaccharide (LPS) extracts obtained from Treponema hyodysenteriae of serogroups A, B, D and E, and from T. innocens were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit ...
Combs, B., Hampson, D.J., Mhoma, J.R.L.
core  

Useful Buffer and Gel Systems for Polyacrylamide Gel Electrophoresis [PDF]

open access: yes, 1971
Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN)
H. R. Maurer   +3 more
core   +1 more source

GENETIC DIFFERENTIATION OF COTTON CULTIVARS BY POLYACRYLAMIDE GEL ELECTROPHORESIS [PDF]

open access: yesJournal of Central European Agriculture, 2005
Information about genetic diversity and relationships among the breeding materials has a signifi cant impact on crop improvement. Association between parental divergence and progeny performance has not been well documented in cotton.
NAVEED Murtaza   +2 more
doaj  

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim   +4 more
wiley   +1 more source

Polysaccharide as a Separation Medium for Gel Electrophoresis

open access: yesPolysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa   +5 more
doaj   +1 more source

Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia

open access: yesJournal of Lipid Research, 2003
Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the β-position on agarose gels.
Dirk J. Blom   +3 more
doaj   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

open access: yesFEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson   +2 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

open access: yesFEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan   +11 more
wiley   +1 more source

Mass Spectrometry-based Proteomics in Acute Respiratory Distress Syndrome: A Powerful Modality for Pulmonary Precision Medicine

open access: yesChinese Medical Journal, 2016
Objective: Acute respiratory distress syndrome (ARDS) is an acute and lethal clinical syndrome that is characterized by hypoxemic respiratory failure and diffuse alveolar inflammatory damage. This review aimed to search and discuss the mass spectrometry (
Xue-Feng Xu   +4 more
doaj   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

open access: yesFEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang   +10 more
wiley   +1 more source

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