Results 91 to 100 of about 920,072 (304)
Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE
Bio-Protocol, 2018 The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system.
Marjaana Rantala +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis [PDF]
, 1999 Pulsed-field gel electrophoresis (PFGE) was applied as a molecular typing tool for the spirochaete Serpulina hyodysenteriae, the agent of swine dysentery.
Atyeo, R.F. +2 more
core
Distribution of Gc Types in Wuhan [PDF]
Fayixue Zazhi, 1985 The distribution of group-specific component(G) types was examined by disc electrophoresis from 508 unrelated residents in Wuhan. Discontinuous buffer system was used in this investigation. The polyacrylamide concentration used for separating gel was 9%,
LONG YANG YANG QING-EN(DEPARTEMENT OF FORENSIC MEDICINE;WUHAN MEDICAL COLLEGE;WUHAN)
doaj
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Polyacrylamide slab gel electrophoresis: An improved design
, 1979 An apparatus for polyacrylamide slab gel electrophoresis is decribed which combines all parts into one integral unit. It eliminates several steps in the process of sealing, pouring, and setting the gels.
Broadmeadow A.C.C., Wilce P.A.
core +1 more source
CHARACTERISATION OF IBD VIRAL PROTEINS IN SDS POLYACRYLAMIDE GEL ELECTROPHORESIS [PDF]
Journal of Veterinary and Animal Sciences, 2004 The field isolates of IBD virus was characterised using protein profile study. Four structural polypeptides of IBD virus with molecular weights of 91 K (VP1), 39 K (VP,), 32 K (VP3), and 28 K (VP4) and the additional polypeptide 47 K (VPX) preceusor ...
M. Parthiban, V. Thiagarajan
doaj
Advanced Functional Materials, EarlyView.Keratin recovered from animal‐hair textile waste is fractionated and blended with cellulose to produce continuously spun hybrid fibers. Selecting the high‐molecular‐weight keratin fraction enables 30 wt.% keratin incorporation while maintaining high strength in dry and wet states.
Mian Zhai +6 more
wiley +1 more source
Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis
BioTechniques, 1998 Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing.
Allan Tereba +2 more
doaj +1 more source