This study reveals that cytoplasmic PABPN1 is essential for mouse oocyte meiotic maturation by coordinating polyadenylation, translation, and degradation of maternal mRNAs. Pabpn1 knockout disrupts CDK1 activation, spindle formation, and chromosome alignment by impairing maturation‐promoting factor (MPF) regulation and BTG4‐mediated deadenylation ...
Xing‐Xing Dai+9 more
wiley +1 more source
Two-Dimensional Polyacrylamide Gel Electrophoresis for Metalloprotein Analysis Based on Differential Chemical Structure Recognition by CBB Dye. [PDF]
Ishikawa J+9 more
europepmc +1 more source
Electrophoresis using polyacrylamide gel as a supporting medium
Goro Mogi
openalex +2 more sources
Sodium dodecyl sulfate electrophoresis of high molecular weight proteins in N, N'‐diallyltartardiamide‐cross‐linked polyacrylamide slab gels [PDF]
Andreas Ziegler, Hans Hengartner
openalex +1 more source
Gallic acid (GA) and copper ions self‐assemble to form nanoparticles, which are then modified with mitochondrial targeting peptides and gap junction modulator. These nanoparticles scavenge mitochondrial reactive oxygen species to induce M2 polarization and enhance intercellular mitochondrial transfer.
Xinzhou Wang+13 more
wiley +1 more source
Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Honoré Bent+4 more
doaj
Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE)in Cell Extracts from Pseudomonas aeruginosa. [PDF]
Pezzoni M, Pizarro RA, Costa CS.
europepmc +1 more source
Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA
Rupert De Wächter, Walter Fiers
openalex +2 more sources
The study develops engineered exosomes (EmDEX@GA) for locoregional immunomodulation of tumor‐draining lymph node (TDLN). EmDEX@GA can actively target to TDLN and remodel its immunosuppressive microenvironment through the synergistic effects of PD‐1/PD‐L1 blockade and STING pathway activation, subsequently enhance anti‐tumor immunity and suppress tumor ...
Yizhen Wang+12 more
wiley +1 more source
Identification of dysregulation of atrial proteins in rats with chronic obstructive apnea using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. [PDF]
Lux JC+6 more
europepmc +1 more source