Blue-native PAGE in plants: a tool in analysis of protein-protein interactions [PDF]
Plant Methods, 2005 Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey +2 more
doaj +3 more sources
Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]
Journal of Mathematical and Fundamental Sciences, 2019 . Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj +2 more sources
The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
Biochemical Journal, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
Evans, RW, Williams, J
core +6 more sources
Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Mass Spectrometry Reviews 28, 5 (2009) 816-43, 2009 Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold +225 more
core +4 more sources
Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE [PDF]
BioTechniques, 2014 Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second ...
Christopher J. Buehl +6 more
doaj +2 more sources
Advanced Biomedical Research, 2015 Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand +5 more
doaj +2 more sources
The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
doaj +1 more source
Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]
Pesquisa Veterinária Brasileira, 2021 : Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo +4 more
doaj +1 more source
A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels
BioTechniques, 2023 Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez +5 more
doaj +1 more source
Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
doaj +1 more source