Results 11 to 20 of about 509,057 (336)

The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]

open access: yesBiochemical Journal, 1980
The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to ...
Evans, RW, Williams, J
openaire   +6 more sources

Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]

open access: yesJournal of Mathematical and Fundamental Sciences, 2019
. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj   +2 more sources

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

open access: yesAl-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed   +2 more
doaj   +1 more source

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

open access: yes, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő   +3 more
core   +1 more source

Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling [PDF]

open access: yesArchives of Biological Sciences, 2011
An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange ...
Asaduzzaman A.K.M.   +2 more
doaj   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

open access: yes, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

open access: yes, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

open access: yesBioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

SDS Agarose Gels for Analysis of Proteins

open access: yesBioTechniques, 1998
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
doaj   +1 more source

Genomic and proteomic profiling for cancer diagnosis in dogs [PDF]

open access: yes, 2016
Global gene expression, whereby tumours are classified according to similar gene expression patterns or ‘signatures’ regardless of cell morphology or tissue characteristics, is being increasingly used in both the human and veterinary fields to assist in ...
Morris, Joanna S.
core   +1 more source

Home - About - Disclaimer - Privacy