Results 31 to 40 of about 693,927 (396)
A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome [PDF]
, 2015 Medicago truncatula leaves were used as the experimental materials. Total proteins of leaves were extracted by trichloracetic acid (TCA)-acetone method and proteins had a better separation using gel strips, forming an immobilized non-linear 3 to 10 pH ...
Dong, Wen +7 more
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Nucleic Acids Research, 1981 We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous
M. Fried, D. Crothers
semanticscholar +1 more source
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source
Isolation and properties of a lectin from the seeds of Mimosa invisa L. [PDF]
, 1987 A lectin has been purified from the seeds ofMimosa invisa L. by gel filtration and preparative Polyacrylamide gel electrophoresis. The purified lectin was homogeneous as judged by analytical Polyacrylamide gel electrophoresis, immunodiffusion and ...
Chandrika, R., Shaila, M. S.
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Optimization of microsatellite DNA Gelred fluorescence imaging technology [PDF]
, 2016 Gelred fluorescent dye has broader prospects of application in DNA experiment because of its high sensitivity, security and stability. In order to explore the best microsatellite DNA Gelred imaging technology, this study compared its dosage by using ...
Da-li, Fu +4 more
core +2 more sources
Study of total seed storage protein in indigenous Brassica species based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [PDF]
, 2016 Genetic diversity was studied in 234 accessions of locally collected Brassica species for total seed protein content through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). These accessions were collected from different locations of
Akmal, M +6 more
core +2 more sources
Non-affine displacements in flexible polymer networks [PDF]
Macromolecules 44, 1671 (2011), 2010 The validity of the affine assumption in model flexible polymer networks is explored. To this end, the displacements of fluorescent tracer beads embedded in polyacrylamide gels are quantified by confocal microscopy under shear deformation, and the deviations of these displacements from affine responses are recorded.
arxiv +1 more source
Conducting polymer electrodes for gel electrophoresis.
PLoS ONE, 2014 In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that Ď-conjugated polymers such as poly(3,4-
Katarina Bengtsson +2 more
doaj +1 more source
Purification and characterisation of a carboxylesterase from the latex ofSynadenium grantii Hook, âfâ [PDF]
, 1987 The latex ofSynadenium grantii was found to contain esterolytic activity. Polyacrylamide gel electrophoretic study coupled with substrate and inhibitor specificity studies revealed the presence of multiple forms of carboxylesterases and cholinesterases ...
Govardhan,, L. +3 more
core +1 more source
The repton model of gel electrophoresis [PDF]
Physica A 244, 25 (1997), 1996 We discuss the repton model of agarose gel electrophoresis of DNA. We review previous results, both analytic and numerical, as well as presenting a new numerical algorithm for the efficient simulation of the model, and suggesting a new approach to the model's analytic solution.
arxiv +1 more source