Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis [PDF]
, 2005 A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were ...
Chhatpar, HS, Gohel, Vipul, Vyas, Pranav
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How to use 2D gel electrophoresis in plant proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 1072 (2014)
43-50, 2013 Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv +1 more source
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Bio-Protocol, 2012 One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
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A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1. [PDF]
PLoS ONE, 2017 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research.
Rahul Mahadev Shelake +4 more
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Evaluation of wheat by polyacrylamide gel electrophoresis [PDF]
, 2012 In this study 15 wheat varieties namely Nacozari-76, Ouqab, Tatar-96, Bakhtawar-92, Yecura-70, Raj, Bakkar, Sulilman-96, Pirsabak-05, Maria, Khyber-87, Fakhr-e-Sarhad, Pirsabak-04, Inqulab-91 and Rawal-87 were evaluated for analysis of variability in ...
Akbar, H +4 more
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DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS [PDF]
Poljoprivreda, 2011 Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated.
Ivica Strelec +2 more
doaj
Novel separation and detection methods of DNA fragments in electrophoresis [PDF]
, 1992 A charge-coupled device (CCD) based electrophoresis system was developed. The system allowed non-destructive, sensitive, and on-line detection of native DNA in slab-gel electrophoresis via ultraviolet absorption measurement. The detection limit of double-
Chan, King Cheung
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Development of a Procedure for Analysis of High Density Lipoprotein Subclasses [PDF]
, 1982 Human serum high density lipoprotein subclasses, HDL2 and HDL3 , were isolated by preparative salt density gradient ultracentrifugation and further analyzed by electrophoresis on a 4 to 15% concentration gradient polyacrylamide gel. The separation of the
Chin, Chan
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Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]
Electrophoresis 18 (31/03/1997) 307-16, 2006 Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv +1 more source
Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv +1 more source