Results 51 to 60 of about 722,396 (378)

A role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in Xenopus laevis embryos [PDF]

open access: yes, 1988
During heat shock, Xenopus laevis embryos exhibit an increase in the rate of accumulation of lactate and a loss of ATP relative to non-heat- shocked control embryos.
Browder, Leon W., Nickells, Robert W.
core   +2 more sources

Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cells

open access: yesFEBS Letters, EarlyView.
Imeglimin, at clinically relevant concentrations, inhibits vesicular ATP accumulation and release from hepatic stellate cells, thereby attenuating purinergic signaling and reducing fibrogenic activation. This mechanism reveals a newly identified antifibrotic action of imeglimin beyond glycemic control.
Seiji Nomura   +8 more
wiley   +1 more source

Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and ...
Aleksander F. Sikorski
doaj   +1 more source

Performance comparison of gel and capillary electrophoresis-based microsatellite genotyping strategies in a population research and kinship testing framework

open access: yesBMC Research Notes, 2021
Objective The advancement of molecular techniques in an era in which high-throughput sequencing has revolutionized biology renders old-fashioned alternatives to high-throughput methods obsolete.
Julissa J. Sánchez-Velásquez   +3 more
doaj   +1 more source

A simple method of drying polyacrylamide slab gels that eliminates cracking

open access: yesBioTechniques, 2021
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
doaj   +1 more source

Characterization of the Lipopolysaccharide from a \u3cem\u3eRhizobium phaseoli\u3c/em\u3e Mutant that is Defective in Infection Thread Development [PDF]

open access: yes, 1987
The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate ...
Carlson, Russell W.   +4 more
core   +1 more source

Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2 [PDF]

open access: yes, 1998
A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way ...
Keeley, A, Oram, M, Tsaneva, IR
core   +2 more sources

Cancer cell death induced by the NAD antimetabolite Vacor discloses the antitumor potential of SARM1

open access: yesFEBS Letters, EarlyView.
Vacor, a compound converted into the toxic metabolite Vacor adenine dinucleotide (VAD) by the nicotinamide salvage pathway enzymes NAMPT and NMNAT2, exhibits antitumor activity by inducing rapid and complete NAD depletion. We report that Vacor toxicity is limited to cell lines expressing high levels of SARM1, a NAD glycohydrolase.
Giuseppe Ranieri   +5 more
wiley   +1 more source

Detection of rotavirus genome by new silver staining method

open access: yesمجله دانشگاه علوم پزشکی گرگان, 2014
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj  

Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

open access: yesPlant Methods, 2005
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey   +2 more
doaj   +1 more source

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