Detection of rotavirus genome by new silver staining method
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj
Blue-native PAGE in plants: a tool in analysis of protein-protein interactions
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey +2 more
doaj +1 more source
A simple method of drying polyacrylamide slab gels that eliminates cracking
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
doaj +1 more source
Biologic activity in a fragment of recombinant human interferon α [PDF]
To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and ...
Ackerman, Samuel K. +4 more
core
Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography [PDF]
Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC).
Abdullah, Norhani +4 more
core +1 more source
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima +4 more
wiley +1 more source
Elution of proteins from polyacrylamide eels: a simple and economic procedure
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj +1 more source
Diversity and Dynamics of Indigenous \u3cem\u3eRhizobium japonicum\u3c/em\u3e Populations [PDF]
A simple method, based upon the separation of cellular proteins by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, has been devised for distinguishing between isolates of Rhizobium japonicum.
Brill, Winston J., Noel, K. Dale
core +1 more source
Modulation of apoprotein E secretion in response to receptor-mediated endocytosis in resident and inflammatory macrophages. [PDF]
We have determined the effect of various endocytic ligands on the secretion of ApoE by macrophages. ApoE was a major secreted protein of resident macrophages, but BCG-activated macrophages secreted little ApoE and periodate-elicited macrophages secreted ...
Takemura, R, Werb, Z
core +2 more sources
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid +3 more
wiley +1 more source

