Results 51 to 60 of about 541,655 (330)

GDP‐fucose transporter SLC35C1: a potential regulatory role in cytosolic GDP‐fucose and fucosylated glycan synthesis

FEBS Open Bio, EarlyView.
The inactivation of SLC35C1 (GDP‐fucose transporter) and enzymes involved in GDP‐fucose biosynthesis was studied. Fucose supplementation increases the level of GDP‐fucose to abnormal, millimolar values in the absence of the TSTA3 protein and SLC35C1 in contrast to the GMDS/SLC35C1 double mutant.
Edyta Skurska, Mariusz Olczak
wiley   +1 more source

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

FEBS Open Bio, EarlyView.
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Shou Soeda, Melissa Montrose, Akinori Takahashi, Risa Ishida, Sandrine Burriel, Nao Ohmine, Tohru Natsume, Shungo Adachi, Minsoo Kim, Tadashi Yamamoto   +9 more
wiley   +1 more source

GENETIC DIFFERENTIATION OF COTTON CULTIVARS BY POLYACRYLAMIDE GEL ELECTROPHORESIS [PDF]

Journal of Central European Agriculture, 2005
Information about genetic diversity and relationships among the breeding materials has a signifi cant impact on crop improvement. Association between parental divergence and progeny performance has not been well documented in cotton.
NAVEED Murtaza, MOTOMITSU Kitaoka, Ali GHULAM MUHAMMAD   +2 more
doaj  

Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America, 1973
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison
L. R. Orrick, M. Olson, H. Busch
semanticscholar   +1 more source

Microphysiological Glomerular Filtration Barriers: Current Insights, Innovations, and Future Applications

Advanced Biology, EarlyView.
The glomerular filtration barrier (GFB) is the first step of blood filtration by the kidneys. The concerning increase of kidney diseases makes the development of new models essential. In this context, microphysiological glomerular filtration barriers focus on closely reproducing the physiological architecture of the in vivo GFB: podocytes, glomerular ...
Manon Miran, Kieu Ngo, David Buob, Hanna Debiec, Pierre Ronco, Guillaume Perry   +5 more
wiley   +1 more source

Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor

Journal of Mathematical and Fundamental Sciences, 2019
. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj  

Investigating the Cellular Effects of GALC Dosing in Enzyme Replacement Therapy for Krabbe Disease Supports the Role of Nanomedicine

Advanced Biology, EarlyView.
A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso, Sara Carpi, Laura Colagiorgio, Miriam De Sarlo, Mariacristina Gagliardi, Marco Cecchini   +5 more
wiley   +1 more source

Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations

Advanced Functional Materials, EarlyView.
Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou, Lauren Brown, Christopher M. Madl   +2 more
wiley   +1 more source

Polysaccharide as a Separation Medium for Gel Electrophoresis

Polysaccharides
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecules.
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta   +5 more
doaj   +1 more source

Cell Membrane Vesicle Camouflaged Artificial Cells

Advanced Functional Materials, EarlyView.
Artificial cells camouflaged with a cell membrane vesicle coating are able to assemble into synthetic aggregates that exhibit rudimentary communication capabilities. Additionally, when these artificial cells are equipped with antioxidant capabilities, they are able to protect the intracellular homeostasis in HepG2 cells present in semi‐synthetic ...
Paula De Dios Andres, Stefan Pendlmayr, Noga Gal, Cathrine Abild Meyer, Cecilie Ryberg, Olav Vestrheim, Noel Angelo Kalacas, Fatih Demir, Markus M. Rinschen, Karsten Haupt, Rajeshwar Prosad Mookerjee, Brigitte Städler   +11 more
wiley   +1 more source