Electrophoretic separation of proteins [PDF]
There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core
Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography [PDF]
Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC).
Abdullah, Norhani+4 more
core +1 more source
The Whereabouts of 2D Gels in Quantitative Proteomics [PDF]
Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series.
arxiv +1 more source
FAM136A depletion upregulated ROS production, reduced mitochondrial membrane potential (ΔΨ) and ATP production, and upregulated expression of PCK1, PCK2, HMGCS1, and HMGCS2. The expression of both TOMM22 and TOMM20 was also upregulated. FAM136A depletion reduced HCCS that produce holocytochrome c by combining heme to apocytochrome c, and reduced the ...
Yushi Otsuka, Masato Yano
wiley +1 more source
Detection of rotavirus genome by new silver staining method
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj
A simple method of drying polyacrylamide slab gels that eliminates cracking
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
doaj +1 more source
Two Dimensional Electrophoresis of Galactosidase Relating to the Disappearance of Bombyx Lectin Activity [PDF]
"Two dimensional polyacrylamide gel electroporesis (2 D-PAGE) analysis on the haemolymph of Bombyx mori was performed using the Mini-PROTEAN mini tube gel two dimensional polyacrylamide gel electrophoresis system (Bio-Rad Laboratories, Inc.).
Yasuo Kato, カトウ ヤスオ
core +1 more source
A biosensor based on magnetoelastic waves for detection of antibodies in human plasma for COVID-19 serodiagnosis [PDF]
The study proposes a new efficient wireless biosensor based on magnetoelastic waves for the detection of antibodies in human plasma, aiming at the serological diagnosis of COVID-19. The biosensor was functionalized with the N antigen - nucleocapsid phosphoprotein of the SARS-CoV-2 virus. Validation analyses, by sodium dodecyl-sulfate polyacrylamide gel
arxiv +1 more source
A potential tumor suppressor role of PLK2 in glioblastoma
PLK2 was consistently downregulated in GBM tissues. Overexpression of PLK2 in GBM cell lines U87MG and U251 reduced their tumorigenic potential and enhanced cell cycle arrest and apoptosis. Suggesting that PLK2 overexpression could potentially be leveraged as a therapeutic strategy to inhibit tumor progression and enhance apoptosis, providing new ...
Xiangping Xia+5 more
wiley +1 more source
Elution of proteins from polyacrylamide eels: a simple and economic procedure
A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
doaj +1 more source