Results 71 to 80 of about 65,820 (278)

Alternative Polyadenylation in Human Diseases

Endocrinology and Metabolism, 2017
Varying length of messenger RNA (mRNA) 3'-untranslated region is generated by alternating the usage of polyadenylation sites during pre-mRNA processing. It is prevalent through all eukaryotes and has emerged as a key mechanism for controlling gene expression.
Jae-Woong Chang, Hsin-Sung Yeh, Jeongsik Yong   +2 more
openaire   +4 more sources

Comprehensive Polyadenylation Site Maps in Yeast and Human Reveal Pervasive Alternative Polyadenylation [PDF]

Cell, 2010
The emerging discoveries on the link between polyadenylation and disease states underline the need to fully characterize genome-wide polyadenylation states. Here, we report comprehensive maps of global polyadenylation events in human and yeast generated using refinements to the Direct RNA Sequencing technology.
Ozsolak, Fatih, Kapranov, Philipp, Foissac, Sylvain, Kim, Sang Woo, Fishilevich, Elane, Monaghan, A. Paula, John, Bino, Milos, Patrice M.   +7 more
openaire   +4 more sources

Synthesis of 2‐Substituted Adenosine Triphosphate Derivatives and their use in Enzymatic Synthesis and Postsynthetic Labelling of RNA

ChemBioChem, EarlyView.
A series of 2‐substituted adenosine 5′‐O‐triphosphate derivatives is synthesized and tested as substrates for polymerases. In vitro transcription with T7 RNA polymerase, primer extension with engineered DNA polymerases, or 3′‐labelling with poly(A) polymerase are used for enzymatic synthesis of base‐modified RNA.
Ugnė Šinkevičiūtė, Tania Sanchez‐Quirante, Samanta Rožánková, Lenka Poštová Slavětínská, Veronika Raindlová, Michal Hocek   +5 more
wiley   +1 more source

Analysis Polyadenylation Signal Usage in Sus scrofa

Animals, 2022
RNA polyadenylation is an important step in the messenger RNA (mRNA) maturation process, and the first step is recognizing the polyadenylation signal (PAS).
Yuting Zhang, Jingwen Song, Min Zhang, Zhongyuan Deng   +3 more
doaj   +1 more source

Mechanisms and Consequences of Alternative Polyadenylation [PDF]

Molecular Cell, 2011
Alternative polyadenylation (APA) is emerging as a widespread mechanism used to control gene expression. Like alternative splicing, usage of alternative poly(A) sites allows a single gene to encode multiple mRNA transcripts. In some cases, this changes the mRNA coding potential; in other cases, the code remains unchanged but the 3' UTR length is ...
James L. Manley, Kensei Nishida, Dafne Campigli Di Giammartino   +2 more
openaire   +3 more sources

A novel transgenic reporter of extracellular acidification in zebrafish elucidates skeletal muscle T‐tubule pH regulation

Developmental Dynamics, EarlyView.
Abstract Disruption of extracellular pH and proton‐sensing can profoundly impact cellular and protein functions, leading to developmental defects. To visualize changes in extracellular pH in the developing embryo, we generated a zebrafish transgenic line that ubiquitously expresses the ratiometric pH‐sensitive fluorescent protein pHluorin2, tethered to
Leif R. Neitzel, Maya Silver, Aaron H. Wasserman, Samantha Rea, Charles C. Hong, Charles H. Williams   +5 more
wiley   +1 more source

Sense-oriented AluYRa1 elements provide a lineage-specific transcription environment for polyadenylation

Scientific Reports, 2021
Transposable elements cause alternative splicing (AS) in different ways, contributing to transcript diversification. Alternative polyadenylation (APA), one of the AS events, is related to the generation of mRNA isoforms in 70% of human genes.
Hyeon-Mu Cho, Se-Hee Choe, Young-Hyun Kim, Hye-Ri Park, Hee-Eun Lee, Ja-Rang Lee, Sang-Je Park, Jae-Won Huh   +7 more
doaj   +1 more source

Cytoplasmic Polyadenylation in Development and Beyond [PDF]

Microbiology and Molecular Biology Reviews, 1999
SUMMARY Maternal mRNA translation is regulated in large part by cytoplasmic polyadenylation. This process, which occurs in both vertebrates and invertebrates, is essential for meiosis and body patterning. In spite of the evolutionary conservation of cytoplasmic polyadenylation, many of the cis elements and trans-acting factors ...
openaire   +4 more sources

Comprehensive Characterization of Alternative Polyadenylation in Human Cancer.

Journal of the National Cancer Institute, 2018
Background Alternative polyadenylation (APA) is emerging as a major post-transcriptional mechanism for gene regulation, and dysregulation of APA contributes to several human diseases.
Yu Xiang, Youqiong Ye, Y. Lou, Yang Yang, Chunyan C Cai, Zhao Zhang, Tingting W. Mills, Ning-yuan Chen, Yoon-Jee Kim, Fatma Muge Ozguc, L. Diao, H. Karmouty-quintana, Yang Xia, R. Kellems, Zheng Chen, M. Blackburn, Seung-Hee Yoo, A. Shyu, G. Mills, Leng Han   +19 more
semanticscholar   +1 more source

Multi‐microRNA diagnostic panel for heart failure with preserved ejection fraction in preclinical and clinical settings

ESC Heart Failure, EarlyView.
Abstract Aims Heart failure with preserved ejection fraction (HFpEF) is a complex syndrome accounting for half of heart failure cases. Although natriuretic peptides are the most accepted and extensively used biomarkers for heart failure, their diagnostic accuracy for HFpEF remains debatable.
Reza Parvan, Natale Rolim, Andreas B. Gevaert, Alessandro Cataliotti, Emeline M. van Craenenbroeck, Volker Adams, Ulrik Wisløff, Gustavo Jose Justo Silva, on behalf of the OptimEx Study Group   +8 more
wiley   +1 more source