Results 91 to 100 of about 92,028 (242)

Regulation of PHOX2B gene expression by the long non‐coding natural antisense RNA PHOX2B‐AS1

The FEBS Journal, EarlyView.
PHOX2B is a transcription factor essential for autonomic nervous system development. We identify and characterize PHOX2B‐AS1, a human long non‐coding antisense transcript at the PHOX2B locus, along with its murine counterpart. Our findings reveal bidirectional transcription and reciprocal regulation: PHOX2B activates PHOX2B‐AS1, whereas PHOX2B‐AS1 ...
Simona Di Lascio, Ana Lucia Cuadros Gamboa, Martina Bertocchi, Filippo Chiesa, Francesca Cargnin, Ettore Mosca, Paride Pelucchi, Viviana Tritto, Stefania Corti, Isabella Ceccherini, Paola Riva, Roberta Benfante, Diego Fornasari   +12 more
wiley   +1 more source

Uncovering a previously unknown function of polyphosphate in polyadenylated RNA‐induced amyloidogenesis of Hfq

The FEBS Journal, EarlyView.
Polyphosphate interaction with Hfq and RNA drives phase separation and amyloid self‐assembly, uncovering a previously unrecognized role for polyP in bacterial stress responses. Our findings reveal new insights into the molecular mechanisms of RNA‐mediated phase separation, highlighting the role of polyP as a key modulator of this process. Polyphosphate
Kevin Mosca, Florian Turbant, Sambhasan Banerjee, Frank Wien, Richard R. Sinden, Véronique Arluison   +5 more
wiley   +1 more source

Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]

, 2014
Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R, Gschweng, Eric H, Kohn, Donald B, Lill, Georgia R   +3 more
core   +1 more source

Post‐transcriptional regulatory networks: The dynamic interplay of RNA‐binding proteins

The FEBS Journal, EarlyView.
Post‐transcriptional gene expression regulation is controlled by RNA‐binding proteins (RBPs), which influence many processes including splicing, localization, translation, and decay. Rather than acting alone, RBPs bind RNAs combinatorially, forming complex, context‐dependent regulatory networks.
Lena A. Street, Marko Jovanovic, Eugenio F. Fornasiero   +2 more
wiley   +1 more source

Rex1p Deficiency Leads to Accumulation of Precursor Initiator tRNA\u3csup\u3eMet\u3c/sup\u3e and Polyadenylation of Substrate RNAs in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e [PDF]

, 2009
A synthetic genetic array was used to identify lethal and slow-growth phenotypes produced when a mutation in TRM6, which encodes a tRNA modification enzyme subunit, was combined with the deletion of any non-essential gene in Saccharomyces cerevisiae.
Anderson, James T., Boone, Charles, Brost, Renee L., Costanzo, Michael, Ozanick, Sarah G., Wang, Xuying   +5 more
core   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Evaluating Malat1-derived 3’end sequences for functional transgene expression in human cells using synthetic mRNA delivery

RNA Biology
The poly(A) tail is a key structural element found in almost all mammalian messenger RNAs, required for stabilizing the transcript and enhancing translation.
Julia Rosemann, Gwendolin Thordis Jahr, Jana Macho, Monika Haemmerle, Tony Gutschner   +4 more
doaj   +1 more source

PlantCTCIP: Chromatin Interaction Prediction Using Convolutional Neural Network and Transformer in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT Chromatin interactions establish spatial proximity between distant regulatory elements and their target genes, significantly influencing gene expression, and phenotypic traits. In this study, we present a plant chromatin interaction prediction model called PlantCTCIP based on Convolutional Neural Networks and Transformer.
Zhenye Wang, Siyu Zhou, Ze Guo, Zilan Ning, Jiaqi Cai, Ran Zhao, Ao Xie, Quan Li, Jiangling Zhang, Yongsheng Zhao, Peizhang Li, Haiping Si, Jianbing Yan, Yong Peng, Jianxiao Liu   +14 more
wiley   +1 more source

Foxg1 and companions: Not only transcription factors

Neural Regeneration Research
Moving from the most recent results on Foxg1 biology, we first summarize the available information on some special pleiotropic effectors of neurodevelopmental interest, involved in controlling both transcription and post-transcriptional steps of gene ...
Antonello Mallamaci, Osvaldo Artimagnella, Gabriele Liuzzi   +2 more
doaj   +1 more source

Expression of a human cartilage procollagen gene (COL2A1) in mouse 3T3 cells. [PDF]

, 1991
Expression in a recombinant system has been difficult to obtain for any of the major fibrillar collagens that require processing by eight or more post-translational enzymes.
Ala-Kokko, Leena, Hyland, James, Jimenez, Sergio A., Kivirikko, Kari I., Prockop, Darwin J., Smith, Carol   +5 more
core   +1 more source