Results 111 to 120 of about 66,094 (222)

Optimization of Conditions for Production of Soluble E. coli Poly(A)-Polymerase for Biotechnological Applications

Biology
Poly(A) polymerase (PAP 1) from Escherichia coli is the primary enzyme responsible for synthesizing poly(A) tails on RNA molecules, signaling RNA degradation in bacterial cells.
Igor P. Oscorbin, Maria S. Kunova, Maxim L. Filipenko   +2 more
doaj   +1 more source

U1 snRNP-Dependent Suppression of Polyadenylation: Physiological Role and Therapeutic Opportunities in Cancer

International Journal of Cell Biology, 2013
Pre-mRNA splicing and polyadenylation are critical steps in the maturation of eukaryotic mRNA. U1 snRNP is an essential component of the splicing machinery and participates in splice-site selection and spliceosome assembly by base-pairing to the 5 ...
Lee Spraggon, Luca Cartegni
doaj   +1 more source

Interplay between Alternative Splicing and Alternative Polyadenylation Defines the Expression Outcome of the Plant Unique OXIDATIVE TOLERANT-6 Gene

Scientific Reports, 2017
Pre-mRNA alternative splicing and alternative polyadenylation have been implicated to play important roles during eukaryotic gene expression. However, much remains unknown regarding the regulatory mechanisms and the interactions of these two processes in
Qingshun Q. Li, Zhaoyang Liu, Wenjia Lu, Man Liu   +3 more
doaj   +1 more source

A Comprehensive RNA-seq Analysis of Human Bocavirus 1 Transcripts in Infected Human Airway Epithelium

Viruses, 2019
Human bocavirus 1 (HBoV1) infects well-differentiated (polarized) human airway epithelium (HAE) cultured at an air-liquid interface (ALI). In the present study, we applied next-generation RNA sequencing to investigate the genome-wide transcription ...
Wei Zou, Min Xiong, Xuefeng Deng, John F. Engelhardt, Ziying Yan, Jianming Qiu   +5 more
doaj   +1 more source

Transcriptional termination and coupled polyadenylation in vitro.

, 2000
Using a coupled, in vitro transcription and polyadenylation system we have investigated the molecular mechanism of transcriptional termination by RNA polymerase II (PolII).
Yonaha, M, Proudfoot, Nicholas
core   +1 more source

Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases. [PDF]

Nucleic Acids Res, 2020
Kaczmarek Michaels K, Mohd Mostafa S, Ruiz Capella J, Moore CL.   +3 more
europepmc   +1 more source

Transcription-dependent polyadenylation signal activity contributes to transcriptomic regulation during Forskolin-induced BeWo cell syncytialization

Frontiers in Cell and Developmental Biology
Polyadenylation, a core transcriptional step governed by polyadenylation signals (PAS), functionally interacts with transcription. Nevertheless, how PAS sequences respond to variable transcriptional output remains poorly characterized. Here, we precisely
Juan Liu, Guolin Ye, Guolin Ye, Xin Liu, Xin Liu, Haiming Jing, Haiming Jing, Shan Gao, Shan Gao, Zhongyuan Deng, Junyu Ning, Junyu Ning   +11 more
doaj   +1 more source

Multiple mechanisms of polyadenylation site choice in male germ cells [PDF]

, 2007
Polyadenylation, the process involved in the formation of the poly(A) tail at the 3¡¬ end of eukaryotic messenger RNAs (mRNAs), has been traditionally described as a two step process.
McMahon, K. Wyatt
core  

Regulation of polyadenylation site choice in plant nuclear mRNA

Environmental and Experimental Botany
The polyadenylation machinery plays a critical role in the processing of pre-mRNA into mature mRNA. This process involves a large multiprotein complex that recognizes polyadenylation signals and determines the polyadenylation sites (PASs).
Wei Zeng, Xiutao Wang, Qiying Zhou, Shengli Jing   +3 more
doaj   +1 more source

Polyadenylation sites.

, 2013
(A) Histogram of clustered polyadenylation sites (PACs) and their normalized position on ORFs. On the x-axis, 0 and 1 refer to the first and last base of the ORF. Only polyadenylation sites of the sense direction with respect to the ORF are shown.
Oscar Franzén (172092), Björn Andersson (2663065), Elin Einarsson (396251), Johan Ankarklev (139024), Marcela Ferella (38642), Jon Jerlström-Hultqvist (260683), Staffan G. Svärd (134025)   +6 more
core   +1 more source