Results 111 to 120 of about 815,697 (298)

Host Cell Protein Challenges in Antibody Processing: Insights Into Persistence and Mitigation Strategies

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Persistence of HCPs remains a major purification challenge in biopharmaceutical manufacturing with direct consequences for product quality, patient safety, and regulatory compliance. Over the last two decades, deeper insights into the mechanisms driving HCP persistence have clarified why certain proteins evade removal during purification ...
Younghoon Oh, Andrew Cooke, Harshal Soni, Susan Liu, Mamadou Bah, Brianna Bellis, Laura Smith, Michael Capaldi, Nicholas Levy, Erik Wilhelm, Kristopher Barnthouse   +10 more
wiley   +1 more source

47 | Dual etiology of chronic hypercobalaminemia: macro-vitamin b12 interference and inflammatory protein overexpression in a patient with sjögren’s syndrome

Geriatric Care
Persistent hypercobalaminemia often triggers extensive investigations to rule out malignancies. However, elevated levels can result from analytical interference or increased cobalamin-binding proteins.
Segreteria SIGOT
doaj  

Core Cross-Linked Polyethylene Glycol Oleate Micelles as Novel Drug Carriers

, 2011
Polyethylene Glycol Oleate was synthesized by esterification of polyethylene glycol and Oil acid using DMAP as a catalyst. The double bonds of the product in the core of micelles were cross-linked by the initiation of (NH4)2S2O8 during the micelles ...
Bao Qi Wang, Jun Zhao, Zhao Shan Cui, Yan Ling Guo   +3 more
core   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation

Journal of Translational Medicine, 2019
Background Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative
N. García-Romero, R. Madurga, G. Rackov, I. Palacín-Aliana, R. Núñez-Torres, A. Asensi-Puig, J. Carrión-Navarro, S. Esteban-Rubio, H. Peinado, A. González-Neira, V. González-Rumayor, C. Belda-Iniesta, A. Ayuso-Sacido   +12 more
doaj   +1 more source

Microfiltration (MF) and Ultrafiltration (UF) for Sewage and Surface Water Concentration for SARS‐CoV‐2 and Other Enveloped Viruses' Detection in Wastewater‐Based Epidemiology (WBE)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT This study objective was to evaluate hollow fiber membranes for concentration of enveloped enteric viruses in sewage and surface water samples. Microfiltration (MF, 0.2 µm nominal pore size) and ultrafiltration (UF, 100 kDa molecular weight cut‐off) hollow fiber membranes were compared for the concentration of the bovine respiratory syncytial ...
Rodrigo Almeria Ragio, Rodrigo de Freitas Bueno, Roseli Frederigi Benassi, Eduardo Lucas Subtil   +3 more
wiley   +1 more source

Green-Emitting Gd3Ga5O12: Tb3+ Nanoparticles Phosphor: Synthesis, Structure, and Luminescence

Nanoscale Research Letters, 2017
Nano- and microceramics of Gd3Ga5O12 garnet doped with 1 mol % Tb3+ ions were synthesized via co-precipitation and high-temperature solid-state reaction methods.
A. Luchechko, L. Kostyk, S. Varvarenko, O. Tsvetkova, O. Kravets   +4 more
doaj   +1 more source

Process Analytical Technology (PAT) for Continuous Purification of mRNA via Precipitation and Tangential Flow Filtration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Continuous downstream processing of messenger ribonucleic acid (mRNA) therapeutics requires purification strategies that are scalable, robust, and capable of removing product‐ and process‐related impurities from in vitro transcription mixtures.
Rajan R. Bhawnani, Maria del Carme Pons Royo, Rohan Kadambi, Andrew Hatas, Steve Burcat, Tyler Arnold, Hasan Al‐Mahayni, Richard D. Braatz, Allan S. Myerson   +8 more
wiley   +1 more source

The influences of the concentrations of “green capping agents” as stabilizers and of ammonia as an activator in the synthesis of ZnS nanoparticles and their polymer nanocomposites

Green Processing and Synthesis, 2017
The green synthetic route for the synthesis of semiconductor nanoparticles has received special attention recently due to its features, such as low cost and environmental friendliness.
Xaba Thokozani, Moloto Makwena J., Al-Shakban Mundher, Malik Mohammad A., Moloto Nosipho, O’Brien Paul   +5 more
doaj   +1 more source

Synthesis of block copolymer including polyepichlorohydrin and polyethylene glycol by "click" chemistry: evaluation of primary parameters of copolymerization

, 2019
WOS: 000490224300003Synthesis of poly(epichlorohydrin-b-ethylene glycol) block copolymers was obtained via "click" chemistry of polyepichlorohydrin propargyl (PECH-propargyl) and polyethylene glycol azido (PEG-N-3) with different molecular weights.
Öztürk, Temel, Yörümez, Cansu
core   +1 more source