PRINSIP UMUM DAN PELAKSANAAN POLYMERASE CHAIN REACTION (PCR) [General Principles and Implementation of Polymerase Chain Reaction] [PDF]
, 2000 Polymerase Chain Reaction (PCR) is an in vitro technique for the amplification of a specific DNA region without prior transfer into living cells. It is a powerful technique because a million-fold amplification can be achieved only in a few hours. For the
Handoyo, Darmo, Rudiretna, Ari
core
Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition
Molecular Oncology, EarlyView.We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li +10 more
wiley +1 more source
Microbiota of Tayohounta, a fermented baobab flavour food of Benin [PDF]
, 2011 The present work provides data on the microbial composition of Tayohounta, a product of natural fermentation of baobab seed kernels. Samples were collected from 3 different small scale producers from Benin at the end of the fermentation process ...
Chadare, F.J. +5 more
core +2 more sources
Molecular Oncology, EarlyView.Nanosecond infrared laser (NIRL) low‐volume sampling combined with shotgun lipidomics uncovers distinct lipidome alterations in oropharyngeal squamous cell carcinoma (OPSCC) of the palatine tonsil. Several lipid species consistently differentiate tumor from healthy tissue, highlighting their potential as diagnostic markers.
Leonard Kerkhoff +11 more
wiley +1 more source
Viral cultures, Polymerase Chain Reaction Cycle Threshold Values and Viral Load Estimation for SARS-CoV-2 Infectious Potential Assessment in Hematopoietic Stem Cell and Solid Organ Transplant Patients: A Systematic Review [PDF]
, 2022 Tom Jefferson +9 more
openalex +1 more source
Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]
, 1991 We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Kaluzab, Brigitte +4 more
core +1 more source
Molecular Oncology, EarlyView.Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee +8 more
wiley +1 more source
Identifikasi Coxiella Brunetii Menggunakan Pengujian Polymerase Chain Reaction pada Kambing di Kota Kupang [PDF]
, 2014 Identification of Coxiella brunetii using polymerase chain reaction method in goats in Kupang area at Nusa Tenggara Timur had been done. Coxiella burnetii as causative of Q fever zoonosis in human and livestock and become public health problem.
Detha, A. I. (Annytha)
core
FEBS Open Bio, EarlyView.CAF‐mediated immunosuppression in ovarian cancer is driven by IDO1, reducing T‐cell function. Inhibiting IDO1 restores T‐cell proliferation and cytotoxicity, increases cancer cell apoptosis, and may help overcome CAF‐induced immune suppression in high‐grade serous ovarian cancer. Targeting IDO1 may improve antitumor immunity.
Hyewon Lee +3 more
wiley +1 more source
Detecting beef meatball contamination with polymerase chain reaction [PDF]
Biotika, 2017 The objective of the study was to describe how much rat and swine primer developed from cytochrome b could detect rat and pork in processed beef products sold in North Maluku.
Hoda A., Sapsuha Y., Syafie Y.
doaj