Results 81 to 90 of about 1,993,919 (353)

Feasibility of rapid polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus colonization among emergency department patients with abscesses

open access: yesOpen Access Emergency Medicine, 2013
Michael S Pulia,1 Mary Calderone,2 Brad Hansen,2 Christine E Stake,3 Mark Cichon,3 Zhanhai Li,4 Nasia Safdar51Division of Emergency Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA; 2Loyola University Stritch ...
Pulia MS   +6 more
doaj  

Identification of Xiphinema vuittenezi by polymerase chain reaction

open access: yesPlant Protection Science, 2004
So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies.
Shesh Kumari   +2 more
doaj   +1 more source

Transcriptional regulation of neuropeptide receptors underlies context‐dependent adaptation in Drosophila melanogaster

open access: yesFEBS Open Bio, EarlyView.
Under environmental changes, the expression level of neuropeptide (NP) and neuropeptide receptor (NPR) genes changes to confer context‐dependent adaptation to the model organism Drosophila melanogaster. Through finding more regulatory elements in the NPR genes in comparison with their ligands (NPs), we found that NPR‐biased transcriptional regulation ...
SeungHeui Ryu   +6 more
wiley   +1 more source

OVERVIEW OF METHODS AND POLYMERASE-CHAIN REACTION MODELS

open access: yesMedična Informatika ta Inženerìâ, 2015
In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques ...
A. S. Sverstyuk   +2 more
doaj   +1 more source

Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients [PDF]

open access: yes, 2013
OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.
Bellei, Nancy Cristina Junqueira   +5 more
core   +4 more sources

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

open access: yesFEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda   +7 more
wiley   +1 more source

A simple, rapid and economic method for detecting multidrug-resistant tuberculosis

open access: yesBrazilian Journal of Infectious Diseases, 2013
Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 ...
Xia Wang   +5 more
doaj   +1 more source

Design of an innovative polymerase chain reaction device based on buoyancy driven flow [PDF]

open access: yes, 2009
This paper was presented at the 2nd Micro and Nano Flows Conference (MNF2009), which was held at Brunel University, West London, UK. The conference was organised by Brunel University and supported by the Institution of Mechanical Engineers, IPEM, the ...
2nd Micro and Nano Flows Conference (MNF2009)   +4 more
core  

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

open access: yesFEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li   +10 more
wiley   +1 more source

PRINSIP UMUM DAN PELAKSANAAN POLYMERASE CHAIN REACTION (PCR) [General Principles and Implementation of Polymerase Chain Reaction] [PDF]

open access: yes, 2000
Polymerase Chain Reaction (PCR) is an in vitro technique for the amplification of a specific DNA region without prior transfer into living cells. It is a powerful technique because a million-fold amplification can be achieved only in a few hours. For the
Handoyo, Darmo, Rudiretna, Ari
core  

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