Results 81 to 90 of about 1,450,683 (312)

The PTTG1/VASP axis promotes oral squamous cell carcinoma metastasis by modulating focal adhesion and actin filaments

Molecular Oncology, Volume 19, Issue 5, Page 1517-1531, May 2025.
VASP was found to be overexpressed in metastatic oral squamous cell carcinoma (OSCC) tissues. Notably, PTTG1‐ and VASP‐deficient OSCC cells demonstrated suppressed metastatic properties by disrupting the interaction between the cytoskeleton and focal adhesion (FAs) in the filopodia region.
Suyeon Park, Sang Shin Lee, Shihyun Kim, Yeonjun Lee, Gyeonwon Park, Jung Oh Kim, Jongho Choi   +6 more
wiley   +1 more source

Detection of Aneuploidy with Digital PCR [PDF]

arXiv, 2007
The widespread use of genetic testing in high risk pregnancies has created strong interest in rapid and accurate molecular diagnostics for common chromosomal aneuploidies. We show here that digital polymerase chain reaction (dPCR) can be used for accurate measurement of trisomy 21 (Down's Syndrome), the most common human aneuploidy.
arxiv  

Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

Proceedings of the National Academy of Sciences of the United States of America, 1988
Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess ...
U. Gyllensten, H. Erlich
semanticscholar   +1 more source

Inhibition of acyl‐CoA synthetase long‐chain isozymes decreases multiple myeloma cell proliferation and causes mitochondrial dysfunction

Molecular Oncology, EarlyView.
Triacsin C inhibition of the acyl‐CoA synthetase long chain (ACSL) family decreases multiple myeloma cell survival, proliferation, mitochondrial respiration, and membrane potential. Made with Biorender.com. Multiple myeloma (MM) is an incurable cancer of plasma cells with a 5‐year survival rate of 59%.
Connor S. Murphy, Heather Fairfield, Victoria E. DeMambro, Samaa Fadel, Carlos A. Gartner, Michelle Karam, Christian Potts, Princess Rodriguez, Ya‐Wei Qiang, Habib Hamidi, Xiangnan Guan, Calvin P. H. Vary, Michaela R. Reagan   +12 more
wiley   +1 more source

CRISPR targeting of FOXL2 c.402C>G mutation reduces malignant phenotype in granulosa tumor cells and identifies anti‐tumoral compounds

Molecular Oncology, Volume 19, Issue 4, Page 1092-1116, April 2025.
FOXL2 c.402C>G mutation drives granulosa cell tumors. Using CRISPR technology, we selectively corrected this mutation, reducing malignancy and increasing sensitivity to dasatinib and ketoconazole. Transcriptomic changes revealed potential therapeutic targets, demonstrating CRISPR's promise for treating this rare ovarian cancer.
Sandra Amarilla‐Quintana, Paloma Navarro, Iván Hernández, Alejandra Ramos, Ana Montero‐Calle, Pablo Cabezas‐Sainz, Maria J Barrero, Diego Megías, Borja Vilaplana‐Martí, Carolina Epifano, Déborah Gómez‐Dominguez, Sara Monzón, Isabel Cuesta, Laura Sánchez, Rodrigo Barderas, Jesús García‐Donas, Alberto Martín, Ignacio Pérez de Castro   +17 more
wiley   +1 more source

A Public Website for the Automated Assessment and Validation of SARS-CoV-2 Diagnostic PCR Assays [PDF]

arXiv, 2020
Summary: Polymerase chain reaction-based assays are the current gold standard for detecting and diagnosing SARS-CoV-2. However, as SARS-CoV-2 mutates, we need to constantly assess whether existing PCR-based assays will continue to detect all known viral strains.
arxiv  

Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction

J. Comput. Biol., 2005
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance.
Sheng Zhao, R. Fernald
semanticscholar   +1 more source

Clinical significance of stratifying prostate cancer patients through specific circulating genes

Molecular Oncology, Volume 19, Issue 5, Page 1310-1331, May 2025.
We tested a specific panel of genes representative of luminal, neuroendocrine and stem‐like cells in the blood of prostate cancer patients, showing predictive value from diagnosis to late stages of disease. This approach allows monitoring of treatment responses and outcomes at specific time points in trajectories.
Seta Derderian, Edouard Jarry, Arynne Santos, Quentin Vesval, Lucie Hamel, Rafael Sanchez‐Salas, Alexis Rompré‐Brodeur, Wassim Kassouf, Raghu Rajan, Fadi Brimo, Marie Duclos, Armen Aprikian, Simone Chevalier   +12 more
wiley   +1 more source

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

Analytical Chemistry, 2011
Droplet digital polymerase chain reaction (ddPCR) is a new technology that was recently commercialized to enable the precise quantification of target nucleic acids in a sample.
Leonardo B. Pinheiro, V. Coleman, Christopher M. Hindson, J. Herrmann, B. Hindson, Somanath Bhat, K. Emslie   +6 more
semanticscholar   +1 more source

Stroma gene signature predicts responsiveness to chemotherapy in pancreatic ductal adenocarcinoma patient‐derived xenograft models

Molecular Oncology, Volume 19, Issue 4, Page 1075-1091, April 2025.
Pancreatic ductal adenocarcinoma patient‐derived xenografts (PDAC‐PDXs) engrafted orthotopically in the pancreas of immunodeficient mice retain the main genetic and histopathological characteristics of the original human tumors. A 294 stroma gene signature differentiates between PDAC‐PDXs that are responsive to gemcitabine plus nab‐paclitaxel versus ...
Alessia Anastasia, Laura Formenti, Paola Ostano, Lucia Minoli, Andrea Resovi, Lavinia Morosi, Claudia Fioravanti, Edoardo Micotti, Cristina Matteo, Eugenio Scanziani, Giovanna Chiorino, Raffaella Giavazzi, Carmen Ghilardi, Dorina Belotti   +13 more
wiley   +1 more source