Results 31 to 40 of about 144,897 (315)

Detection and characterization of Rickettsial strains in ticks from Sardinia, Italy [PDF]

, 2006
The aim of this study was, firstly, to detect the presence of Rickettsial DNA by PCR and, then, to identify the Rickettsiae species using restriction endonuclease fragment length polymorphism (RFLP) on two amplified ...
Longheu, Carla, Masala, Giovanna, Mura, A. M., Piras, Pierpaola, Satta, Giuseppe, Tola, Sebastiana   +5 more
core  

Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil [PDF]

, 2001
Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum
Adolphe Zeze, Amarger N., Bromfield E.S.P., Chanway C.P., Demezas D.H., Eardly B.D., Harrison S.P., Harrison S.P., Haukka K., Hirsch P.R., J. Peter W. Young, Jones D.G., Kaijalainen S., Kirshtein J.D., Laguerre G., Laguerre G., Laguerre G., Lesley A. Mutch, Leung K., Lieven-Antoniou C.A., Liu W.T., Louvrier P., McDonald I.R., Page R.D.M., Saitou N., Sambrook J., Segovia L., Smalla K., Strain S.R., Sullivan J.T., Turner S.L., Young J.P.W., Young J.P.W., Young J.P.W., Zhou J.Z.   +34 more
core   +1 more source

SPG4 and Dementia: Expanding the Clinical Spectrum

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza, Arun Meyyazhagan, Eliseo Picchi, Gustavo Ribas, Ingrid Faber, Ryosuke Miyamoto, Preethi Basavaraju, Paolo Eusebi, Haripriya Kuchi Bhotla, Mario Stasi, Fabrizio Gaudiello, Francesco Patti, Filippo Maria Santorelli, Marcondes Cavalcante França Jr, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Hélio Afonso Ghizoni Teive, Peter Henry St George‐Hyslop, Toshitaka Kawarai, Antonio Orlacchio   +19 more
wiley   +1 more source

A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento, Daniela F. Santos, Tuane C. R. G. Vieira, Tiago F. Outeiro   +3 more
wiley   +1 more source

IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

Ribogospodarsʹka Nauka Ukraïni, 2013
The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency
Yu. Rud
doaj   +1 more source

Outbreaks of Pneumocystis Pneumonia in 2 Renal Transplant Centers Linked to a Single Strain of Pneumocystis: Implications for Transmission and Virulence [PDF]

, 2017
By restriction fragment length polymorphism analysis, 2 outbreaks of Pneumocystis pneumonia in renal transplant patients in Europe were shown to be caused by the same strain of Pneumocystis; another outbreak in Japan was caused by a different ...
Fehr, Thomas, Gianella, Sara, Gogineni, Emile, Goto, Norihiko, Huber, Charles, Konrad, Regina, Kovacs, Joseph A., Kutty, Geetha, Ma, Liang, Mueller, Nicolas J., Oka, Shinichi, Ripamonti, Chiara, Sassi, Monica, Sing, Andreas, Yazaki, Hirohisa   +14 more
core  

Time‐Dependent Oxidation and Scale Evolution of a Wrought Co/Ni‐Based Superalloy

Advanced Engineering Materials, EarlyView.
This study shows how a new wrought Co/Ni‐based superalloy resists oxidation at 800 ∘$^\circ$C. The oxide scale changes from rough, fast‐growing spinel to a dense, protective chromia–alumina layer. Atom probe analysis reveals tiny refractory‐rich bubbles at the interface that mark the transition to long‐term, diffusion‐controlled protection ...
Cameron Crabb, Zachary T. Kloenne, Samuel R. Rogers, Chi Hang Dominik Kwok, Mark C. Hardy, Michele S. Conroy, David Dye   +6 more
wiley   +1 more source

MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]

, 2002
Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
., Sutarno, A.J, Lymbery, J.M, Cummins, RCA, Thompson   +3 more
core  

Restriction fragment length polymorphism (RFLP) [PDF]

American Journal of Physical Anthropology, 1989
A restriction fragment length polymorphism (RFLP) is defined by an enzyme, a restriction endonuclease, that cuts the doublestranded DNA at a particular sequence of bases, a probe, a labeled, complementary segment of DNA that will anneal to a portion of the digested sample, and a set of variable fragment length bands that appear on a Southern blot.
openaire   +1 more source

Formation of Stable Amorphous Calcium Phosphate and Collagen Assemblies by a Versatile Spray‐Drying Approach

Advanced Functional Materials, EarlyView.
Amorphous calcium phosphate (ACP) microparticles with long‐term and thermal stability are prepared with or without collagen using a scalable one‐pot spray‐drying process. Under simulated physiological conditions, they crystallize into biomimetic bone mineral and, when combined with collagen, form extrudable, fibrillar bone‐like 3D constructs.
Camila Bussola Tovani, Nessma Bennaceur, Celina Benkhellat, Tristan Georges, Clara Doisneau, Vaskar Sardhalia, Thierry Azaïs, Flora Lemaire, Halima Kerdjoudj, Mohamed Selmane, Capucine Sassoye, Marie Albéric, Cédric Boissière, Nadine Nassif   +13 more
wiley   +1 more source