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Solid-phase synthesis of oligoribonucleotides using T4 RNA ligase and T4 polynucleotide kinase
Biochimie, 1995 The concept of solid-phase synthesis of oligoribonucleotides using T4 RNA ligase and T4 polynucleotide kinase has been proposed and tested with model homo-oligoribonucleotides. The method consists of the immobilization of the first oligomer block at the 3'-terminus on a solid support followed by a chain elongation in the 5'-direction with trinucleoside
Lyubov V. Vratskikh +2 more
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Polynucleotide Ligase and φX174 Single Strand Synthesis
Nature New Biology, 1971A DNA ligase mutant of E. coli when infected with φX174 produces linear single strands which appear in an intracellular pool and in phage particles. The linear single strands, which are infectious in a spheroplast assay, seem to be a normal intermediate in progeny DNA synthesis.
R W, Schekman, D S, Ray
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Repair of single strand breaks in transforming DNA by polynucleotide ligase
Biochemical and Biophysical Research Communications, 1968 Jun Takagi +2 more
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Polynucleotide ligase from rat liver after partial hepatectomy
Biochemical and Biophysical Research Communications, 1971Abstract Polynucleotide ligase, which catalyzes the covalent joining of two segments of an interrupted strand in a DNA duplex, was extracted from rat liver. The activity is located in both nuclei and soluble fractions. Its optimal pH was 8.0. The enzyme requires ATP as a cofactor. Its activity was completely dependent on the presence of Mg 2+ .
K, Tsukada, M, Ichimura
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Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4. II
Virology, 1973 Voon Loong Chan, K. Ebisuzaki
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Effect of ATP analogues on T4 polynucleotide ligase
Biochemical and Biophysical Research Communications, 1978Abstract The influence of three ATP analogues, α, β-methylene adenosine 5′-triphosphate, β, γ-methylene adenosine 5′-triphosphate and β, γ-imidoadenosine 5′-triphosphate on the activity of T 4 polynucleotide ligase has been investigated. Only β, γ-imidoadenosine 5′-triphosphate was active in the joining reaction; the rate of catalysis being slightly
A J, Raae, K, Kleppe
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Polynucleotide cellulose as a substrate for a polynucleotide ligase induced by phage T4
Biochemical and Biophysical Research Communications, 1967Abstract An enzyme which joins polydeoxynucleotide strands has been identified in and purified from extracts Escherichia coli infected with phage T4 am N82 and appears to be the same as the ligase described by Weiss and Richardson. A novel method for detecting and assaying such enzymes involves the joining of a strand to one that is ...
N R, Cozzarelli +3 more
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DNA replication in vivo by polynucleotide-ligase defective mutants of T4
Journal of Molecular Biology, 1971 Junko Hosoda, Elaine Mathews
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Partial Characterization of Polynucleotide Ligase from Baby-Hamster Kidney Cells
Biochemical Society Transactions, 1973 HUGH YOUNG +4 more
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Changes in polynucleotide ligase during rat liver regeneration
Biochemical and Biophysical Research Communications, 1974Abstract The specific activity of polynucleotide ligase in rat liver seems to begin to rise at 16 hours after partial hepatectomy (removal of 70% of the liver). The increases reach their maxima about 24 hours after operation, rising to at least 4 to 5 fold normal levels.
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