Results 71 to 80 of about 43,981 (239)

Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs

Bio-Protocol, 2018
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases.
Christian Beren, Katherine Liu, Lisa Dreesens, Charles Knobler, William Gelbart   +4 more
doaj   +1 more source

RNase E biomolecular condensates stimulate PNPase activity

Scientific Reports, 2023
Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins, Dylan T. Tomares, Vidhyadhar Nandana, Jared M. Schrader, W. Seth Childers   +4 more
doaj   +1 more source

Genome wide association studies for acid phosphatase activity at varying phosphorous levels in Brassica juncea L

Frontiers in Plant Science, 2022
Acid phosphatases (Apases) are an important group of enzymes that hydrolyze soil and plant phosphoesters and anhydrides to release Pi (inorganic phosphate) for plant acquisition. Their activity is strongly correlated to the phosphorus use efficiency (PUE)
Priyanka Upadhyay, Mehak Gupta, Simarjeet Kaur Sra, Rakesh Sharda, Sanjula Sharma, Virender K. Sardana, Javed Akhatar, Gurpreet Kaur   +7 more
doaj   +1 more source

A PNPase dependent CRISPR System in Listeria. [PDF]

PLoS Genetics, 2014
The human bacterial pathogen Listeria monocytogenes is emerging as a model organism to study RNA-mediated regulation in pathogenic bacteria. A class of non-coding RNAs called CRISPRs (clustered regularly interspaced short palindromic repeats) has been ...
Nina Sesto, Marie Touchon, José Marques Andrade, Jiro Kondo, Eduardo P C Rocha, Cecilia Maria Arraiano, Cristel Archambaud, Éric Westhof, Pascale Romby, Pascale Cossart   +9 more
doaj   +1 more source

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

mBio, 2022
The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer   +4 more
doaj   +1 more source

The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells

npj Biofilms and Microbiomes, 2023
Biofilms provide an environment that protects microorganisms from external stresses such as nutrient deprivation, antibiotic treatments, and immune defences, thereby creating favorable conditions for bacterial survival and pathogenesis. Here we show that
Ana Patrícia Quendera, Sandra Nunes Pinto, Vânia Pobre, Wilson Antunes, Vasco D. B. Bonifácio, Cecília Maria Arraiano, José Marques Andrade   +6 more
doaj   +1 more source

Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized. [PDF]

, 2018
In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and
Gammage, Payam A, Minczuk, Michal, Moraes, Carlos T   +2 more
core   +1 more source

Identification of Polynucleotide Phosphorylase (PNPase) in Escherichia coli Involved in Persister Formation

bioRxiv, 2018
Despite the identification of many genes and pathways involved in the persistence phenomenon of bacteria, the mechanisms of persistence are not well understood.
Nan Wu, Yumeng Zhang, Shanshan Zhang, Youhua Yuan, Shuang Liu, P. Cui, Wen Zhang, Ying Zhang   +7 more
semanticscholar   +1 more source

Composition and conservation of the mRNA-degrading machinery in bacteria [PDF]

, 2011
RNA synthesis and decay counteract each other and therefore inversely regulate gene expression in pro- and eukaryotic cells by controlling the steady-state level of individual transcripts.
Dharam Singh, Sue Lin-Chao, Vladimir R Kaberdin   +2 more
core   +4 more sources

Resveratrol Attenuates Exercise-Induced Adaptive Responses in Rats Selectively Bred for Low Running Performance

Dose-Response, 2014
Low capacity runner (LCR) rats have been developed by divergent artificial selection for treadmill endurance capacity to explore an aerobic biology-disease connection.
Nikolett Hart, Linda Sarga, Zsolt Csende, Lauren G. Koch, Steven L. Britton, Kelvin J.A. Davies, Zsolt Radak   +6 more
doaj   +1 more source