Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains. [PDF]
Liu B+5 more
europepmc +2 more sources
Human polynucleotide phosphorylase (hPNPase(old-35)): an evolutionary conserved gene with an expanding repertoire of RNA degradation functions. [PDF]
Das SK+6 more
europepmc +3 more sources
A mutant deficient in polynucleotide phosphorylase (PNPase) activity was previously constructed in Enterococcus faecalis 14; a strain producing a leaderless two-peptide enterocin DD14 (EntDD14).
Rabia Ladjouzi+3 more
doaj +1 more source
CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence. [PDF]
Numata S+4 more
europepmc +2 more sources
Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase
The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y Chou, P McPhie, M F Singer
openaire +2 more sources
Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases.
Christian Beren+4 more
doaj +1 more source
RNase E forms a complex with polynucleotide phosphorylase in cyanobacteria via a cyanobacterial-specific nonapeptide in the noncatalytic region. [PDF]
The endoribonuclease RNase E in a range of bacteria has a conserved N-terminal catalytic domain and a less well-conserved C-terminal tail. While the role of the C-terminal tail in the formation of the degradosome, including the interaction with the ...
Zhang JY+6 more
europepmc +2 more sources
Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized. [PDF]
In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and
Gammage, Payam A+2 more
core +1 more source
Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics [PDF]
Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and
Garavaglia, Betiana Soledad+6 more
core +1 more source
S1 and KH domains of polynucleotide phosphorylase determine the efficiency of RNA binding and autoregulation. [PDF]
Wong AG+4 more
europepmc +2 more sources