Results 71 to 80 of about 40,677 (203)
Localization of components of the RNA-degrading machine in Bacillus subtilis
In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa+2 more
doaj +1 more source
Solvation Effects on Hole Mobility in the Poly G/Poly C Duplex [PDF]
Theoretical calculations of solvation contribution to hole energy in a polynucleotide chain give very low hole mobility values at zero temperature, \mu < 10^{-3} cm^2/(V s). We calculated hole mobility at physiological temperature for the Poly G/Poly C DNA duplex, which gave substantially larger mobility values.
arxiv +1 more source
Low capacity runner (LCR) rats have been developed by divergent artificial selection for treadmill endurance capacity to explore an aerobic biology-disease connection.
Nikolett Hart+6 more
doaj +1 more source
Regulation of Escherichia coli Polynucleotide Phosphorylase by ATP [PDF]
Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, processively catalyzes the phosphorolysis of RNA, releasing nucleotide diphosphates, and the reverse polymerization reaction. In Escherichia coli, both reactions are implicated in RNA decay, as addition of either poly(A) or heteropolymeric tails targets ...
M. Del Favero+5 more
openaire +6 more sources
Background The polynucleotide phosphorylase (PNPase) is conserved among both Gram-positive and Gram-negative bacteria. As a core part of the Escherichia coli degradosome, PNPase is involved in maintaining proper RNA levels within the bacterial cell.
Daniel-Timon Spanka+2 more
doaj +1 more source
Pulling hairpinned polynucleotide chains: Does base-pair stacking interaction matter? [PDF]
Force-induced structural transitions both in relatively random and in designed single-stranded DNA (ssDNA) chains are studied theoretically. At high salt conditions, ssDNA forms compacted hairpin patterns stabilized by base-pairing and base-pair stacking interactions, and a threshold external force is needed to pull the hairpinned structure into a ...
arxiv +1 more source
Regulatory Mechanisms of Exoribonuclease PNPase and Regulatory Small RNA on T3SS of Dickeya dadantii
The type III secretion system (T3SS) is an essential virulence factor for many bacterial pathogens. Polynucleotide phosphorylase (PNPase) is one of the major exoribonucleases in bacteria and plays important roles in mRNA degradation, tRNA processing, and
Quan Zeng+3 more
doaj +1 more source
Rat liver mitochondrial polynucleotide phosphorylase
We have recently isolated, partially purified and demonstrated the specificity of a polynucleotide phosphorylase from crude guinea-pig liver nuclei [ 1,2] The enzyme was associated with membrane lipids and catalysed the phosphorolysis of polyribonucleotides to ribonucleoside diphosphates.
Yew Phew See, P. S. Fitt
openaire +3 more sources
Complex Formation Between Polyelectrolytes and Ionic Surfactants [PDF]
The interaction between polyelectrolyte and ionic surfactant is of great importance in different areas of chemistry and biology. In this paper we present a theory of polyelectrolyte ionic-surfactant solutions. The new theory successfully explains the cooperative transition observed experimentally, in which the condensed counterions are replaced by ...
arxiv +1 more source
Mg2+ ion effect on stability of double-stranded popynucleotide formed by polyriboinosinic and polyribocytidilic chains [PDF]
Mg2+ effect on the thermal stability of the double-stranded polynucleotide polyIpolyC (IC) under conditions close to physiological ones (0.1M Na+, pH7) was studied by the differential UV spectroscopy. The initial increase of the ion concentration ([Mg2+]) leads to the increase of the melting temperature (Tm) and to the decrease of the absorption ...
arxiv