Results 101 to 110 of about 17,823 (227)
Base Orientation of Second DNA in RecA·DNA Filaments. Analysis by combination of linear dichroism and small angle neutron scattering in flow-oriented solution [PDF]
, 1998 To gain insight into the mechanism of pairing two complementary DNA strands by the RecA protein, we have determined the nucleobase orientation of the first and the second bound DNA strands in the RecA·DNA filament by combined measurements of linear ...
Ellouze, Christine +5 more
core +1 more source
The Reaction of Formaldehyde with Polynucleotides
Journal of Biological Chemistry, 1961 Formaldehyde has been used extensively for chemically modifying proteins and for inactivating toxins and viruses, although the precise mode of its action is little understood. In the case of tobacco mosaic virus, however, Fraenkel-Conrat (1) and Staehelin (2) have shown that inactivation of the virus is due to interaction of formaldehyde with the amino
Robert Haselkorn, Paul Doty
openaire +3 more sources
ChemSystemsChem, EarlyView.Self‐organisation may result from organic systems fed with free energy provided that reactivity becomes a determining factor. The propensity of carbon to form covalent bonds can give rise to kinetically stable thermodynamically activated compounds possibly undergoing non‐linear kinetic processes.
Robert Pascal
wiley +1 more source
Concentration and isolation of DNA from biological fluids by agarose gel isotachophoresis
BioTechniques, 2005 Isotachophoresis is an electrophoretic method of separation of charged substances. The method is characterized by a discontinuous buffer system, constant velocity of separated molecules, and the distribution of separated components in the form of narrow ...
Valentina N. Kondratova +3 more
doaj +1 more source
A novel DNA vaccine technology conveying protection against a lethal herpes simplex viral challenge in mice. [PDF]
PLoS ONE, 2013 While there are a number of licensed veterinary DNA vaccines, to date, none have been licensed for use in humans. Here, we demonstrate that a novel technology designed to enhance the immunogenicity of DNA vaccines protects against lethal herpes simplex ...
Julie L Dutton +8 more
doaj +1 more source
Derivatization of unprotected polynucleotides
Nucleic Acids Research, 1983 A simple and efficient method for attaching amines to the terminal 5'-phosphate of unprotected oligonucleotides or nucleic acids in aqueous solution is described. The method is applicable to low molecular-weight amines, polypeptides, or proteins. The terminal 5'-phosphate of an oligonucleotide or nucleic acid reacts with a water-soluble carbodiimide in
Barbara C.F. Chu +2 more
openaire +4 more sources
Nickel (II) Ions Interaction with Polynucleotides and DNA of Different GC Composition [PDF]
arXiv, 2009 The goal of the work was to study the role of GC alternative dimmers in the binding of DNA with Ni (II) ions. The method of ultraviolet difference spectroscopy has been applied to investigate Ni (II) ions interactions with DNA extracted from Clostridium perfringens, Mice liver (C3HA line), Calf thymus, Salmon sperm, Herring sperm, E.coli, Micrococcus ...
arxiv
Lack of Z-DNA Conformation in Mitomycin-Modified Polynucleotides Having Inverted Circular Dichroism [PDF]
, 1983 Poly(dG-dC)· poly(dG-dC) and Micrococcus lysodeikticus DNA were modified by exposure to reductively activated mitomycin C, an antitumor antibiotic. The resulting covalent drug-polynucleotide complexes displayed varying degrees of CD inversions, which are
Barton, Jacqueline K. +5 more
core +1 more source
Polynucleotide-Chitosan Complex, an Insoluble but Reactive Form of Polynucleotide.
Chemical and Pharmaceutical Bulletin, 1997 DNA formed an insoluble complex on mixing with chitosan (poly-D-glucosamine) in solution. The DNA content of the complex was about 50% and the DNA remained insoluble in aqueous media of pH 2-7%; e.g., on treatment of the DNA-chitosan complex with phosphate-buffered saline at pH 7 and 37 degrees C for 26 h, the DNA released into the aqueous phase was ...
Takashi Kubo +3 more
openaire +3 more sources
The FEBS Journal, EarlyView.When overexpressed in Staphylococcus aureus, the type I toxins SprA1 and SprA2 form membrane pores, with SprA1 creating stable pores and SprA2 forming transient ones. Both induce concomitant membrane depolarization, ATP release, and growth arrest, while only SprA1 causes membrane permeabilization due to its stable pore formation.
Laurence Fermon +10 more
wiley +1 more source