Results 31 to 40 of about 1,741 (114)

Transfer RNA nucleotidyltransferase repairs all transfer RNAs randomly

open access: yes, 1977
Total Escherichia coli transfer RNA was converted to tRNA-C-C by periodate oxidation and partially repaired with either E. coli or rabbit liver tRNA nucleotidyltransferase. Aminoacylation of the partially repaired molecules indicated that individual tRNA
Deutscher, Murray P, Evans, Jeffrey A
core   +1 more source

[58] Rabbit liver tRNA nucleotidyltransferase

open access: yes, 1974
This chapter describes the procedures for the purification of the enzyme from Escherichia coli, and rabbit liver. This procedure yields a preparation of the enzyme from rabbit liver that is homogeneous and about 30,000-fold purified.
Deutscher, Murray P   +1 more
core   +1 more source

High‐quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures

open access: yesMicrobiologyOpen, Volume 13, Issue 5, October 2024.
In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold   +11 more
wiley   +1 more source

Multilayered proteomics reveals that JAM‐A promotes breast cancer progression via regulation of amino acid transporter LAT1

open access: yesCancer Science, Volume 115, Issue 9, Page 3153-3168, September 2024.
We uncovered junctional adhesion molecule‐A (JAM‐A), one of the transmembrane‐type tight junction proteins (TJPs), promoted breast cancer progression via regulation of amino acid transporter LAT1 using multilayered proteomics. Our findings provide a new insight into a therapeutic strategy, JAM‐A‐targeted therapy ideally combined with LAT1‐targeted ...
Kazufumi Magara   +14 more
wiley   +1 more source

A heterologous system for exploring the localization of Arabidopsis tRNA nucleotidyltransferase [PDF]

open access: yes, 2007
The tRNA nucleotidyltransferase enzyme is crucial for normal tRNA maturation in eukaryotic cells. Sequence analysis revealed a possible nuclear localization signal (KDTKGKSIPVVNHIFKFSMKRK) in the Arabidopsis thaliana tRNA nucleotidyltransferase.
Kanaan, Krystel
core   +1 more source

Polyamine stimulation and cation requirements of rabbit liver tRNA nucleotidyltransferase

open access: yes, 1976
We have examined the cation requirements of rabbit liver tRNA nucleotidyltransferase. The enzyme had an absolute requirement fo a divalent cation which could be satisfied by Mg2+, Mn2+ or Co2+. In contrast to the Escherichia coli enzyme, we have found no
Evans, J A, Deutscher, M P
core   +2 more sources

A physiological role for tRNA nucleotidyltransferase during bacteriophage infection

open access: yes, 1976
Bacteriophage infection of E. coli cells deficient in the enzyme tRNA nucleotidyltransferase ( cca mutants) resulted in greatly decreased production of viable progeny phage compared to wild type cells. This decrease amounted to as much as 90% in the case
Morse, Jeffrey W, Deutscher, Murray P
core   +1 more source

Preparation of Cells Permeable to Macromolecules by Treatment with Toluene: Studies of Transfer Ribonucleic Acid Nucleotidyltransferase

open access: yes, 1974
Transfer ribonucleic acid (tRNA) nucleotidyltransferase was studied after making cells permeable to macromolecules by treatment with toluene. The conditions of toluene treatment necessary for obtaining maximal activity were defined. Toluene treatment was
Deutscher, Murray P
core   +3 more sources

A novel nucleolytic activity associated with rabbit liver tRNA nucleotidyltransferase

open access: yes, 1973
Purified preparations of rabbit liver tRNA nucleotidyltransferase contain a nucleolytic activity which removes terminal CMP residues from tRNA-C-C and tRNA-C-C-C.
Deutscher, Murray P
core   +1 more source

The role of arginine 244 in Candida glabrata tRNA nucleotidyltransferase [PDF]

open access: yes, 2009
The enzyme ATP(CTP):tRNA nucleotidyltransferase is essential in eukaryotes to allow tRNAs to participate in protein synthesis. It adds a specific cytidine-cytidine-adenosine (CCA) sequence to generate positions 74, 75, and 76 at the 3' ends of tRNAs in a
Arthur, Jason
core   +1 more source

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