Results 61 to 70 of about 6,340 (193)

Biochemical Characterization and Elucidation of the Hybrid Action Mode of a New Psychrophilic and Cold-Tolerant Alginate Lyase for Efficient Preparation of Alginate Oligosaccharides

Marine Drugs, 2022
Alginate lyases with unique biochemical properties have irreplaceable value in food and biotechnology industries. Herein, the first new hybrid action mode Thalassotalea algicola-derived alginate lyase gene (TAPL7A) with both psychrophilic and cold ...
Shengsheng Cao, Li Li, Benwei Zhu, Zhong Yao   +3 more
doaj   +1 more source

Alginate-modifying enzymes: Biological roles and biotechnological uses

Frontiers in Microbiology, 2015
Alginate denotes a group of industrially important 1-4-linked biopolymers composed of the C-5-epimers β-D-mannuronic acid (M) and α-L-guluronic acid (G).
Helga eErtesvåg
doaj   +1 more source

Enzymatic depolymerization of alginate by two novel thermostable alginate lyases from Rhodothermus marinus

Frontiers in Plant Science, 2022
Alginate (alginic acid) is a linear polysaccharide, wherein (1→4)-linked β-D-mannuronic acid and its C5 epimer, α-L-guluronic acid, are arranged in varying sequences.
Justyna M. Dobruchowska, Bryndis Bjornsdottir, Olafur H. Fridjonsson, Josef Altenbuchner, Hildegard Watzlawick, Gerrit J. Gerwig, Lubbert Dijkhuizen, Johannis P. Kamerling, Gudmundur O. Hreggvidsson, Gudmundur O. Hreggvidsson   +9 more
doaj   +1 more source

Thiolated Hyaluronic Acid: A Gateway for Targeted Killing of Staphylococcus aureus on the Race for Surface Colonization

Advanced Healthcare Materials, EarlyView.
Thiolated hyaluronic acid (HAMS) synthesized and characterized by NMR, solubility, thiol content, and pKa, is degraded by Staphylococcal hyaluronate lyase but not by mammalian hyaluronidase. Coating polyphosphate–M23 phage endolysin nanoparticles (M23‐PP) with HAMS confers Staphylococcus aureus responsiveness.
Mariana Blanco Massani, Susanne Meile, Annabelle Knoll, David Gintsburg, Ilaria Polidori, Anna Seybold, Débora C. Coraça‐Huber, Martin J. Loessner, Gergely Kali, Mathias Schmelcher, Szczepan Zapotoczny, Andreas Bernkop‐Schnürch   +11 more
wiley   +1 more source

Life Factors and Melanoma: From the Macroscopic State to the Molecular Mechanism

Advanced Science, EarlyView.
Melanoma, an aggressive skin cancer, arises from dynamic interactions between genetic, environmental, and lifestyle factors. This review explores how age, gender, obesity, diet, exercise, smoking, alcohol, UV exposure, circadian rhythms, and medications influence melanoma risk and progression.
Hanbin Wang, Hengxiang Zhang, Yuqi Zhou, Chunying Li, Weinan Guo   +4 more
wiley   +1 more source

Biological Conversion of Formate to Organic Compounds: Toward a Sustainable Formate Bioeconomy

Carbon Energy, EarlyView.
Formate bioconversion plays a crucial role in achieving renewable resource utilization and sustainable development. To tap its full potential, it is important to identify the most appropriate microbial hosts for incorporating formate into building blocks, design the most promising metabolic pathways for transmitting formate into central carbon ...
Jinyi Qian, Tiantian Chai, Chunlei Zhao, Xiulai Chen   +3 more
wiley   +1 more source

Cloning, Expression, and Characterization of a New PL25 Family Ulvan Lyase from Marine Bacterium Alteromonas sp. A321

Marine Drugs, 2019
Ulvan lyases can degrade ulvan to oligosaccharides with potent biological activity. A new ulvan lyase gene, ALT3695, was identified in Alteromonas sp. A321. Soluble expression of ALT3695 was achieved in Escherichia coli BL21 (DE3).
Jian Gao, Chunying Du, Yongzhou Chi, Siqi Zuo, Han Ye, Peng Wang   +5 more
doaj   +1 more source

Gut microbiota‐related modulation of immune mechanisms in post‐infarction remodelling and heart failure

ESC Heart Failure, Volume 12, Issue 2, Page 942-954, April 2025.
Abstract The immune system has long been recognized as a key driver in the progression of heart failure (HF). However, clinical trials targeting immune effectors have consistently failed to improve patient outcome across different HF aetiologies. The activation of the immune system in HF is complex, involving a broad network of pro‐inflammatory and ...
Johann Roessler, Friederike Zimmermann, Bettina Heidecker, Ulf Landmesser, Arash Haghikia   +4 more
wiley   +1 more source

Identification and characterization of a critical loop for the high activity of alginate lyase VaAly2 from the PL7_5 subfamily

Frontiers in Microbiology
As the major component in the cell wall of brown algae, alginates are degradable by alginate lyases via β-elimination. Alginate lyases can be categorized into various polysaccharide lyase (PL) families, and PL7 family alginate lyases are the largest ...
Muxuan Du, Muxuan Du, Xue Li, Weipeng Qi, Yingjie Li, Lushan Wang   +5 more
doaj   +1 more source

Cloning and characterization of a pectin lyase gene from Colletotrichum lindemuthianum and comparative phylogenetic/structural analyses with genes from phytopathogenic and saprophytic/opportunistic microorganisms

BMC Microbiology, 2011
Background Microorganisms produce cell-wall-degrading enzymes as part of their strategies for plant invasion/nutrition. Among these, pectin lyases (PNLs) catalyze the depolymerization of esterified pectin by a β-elimination mechanism.
Lara-Márquez Alicia, Zavala-Páramo María G, López-Romero Everardo, Calderón-Cortés Nancy, López-Gómez Rodolfo, Conejo-Saucedo Ulises, Cano-Camacho Horacio   +6 more
doaj   +1 more source