Lack of Genetic Diversity in Newly Sequenced Porcine Circovirus Type 1 Strains Isolated 20 Years Apart [PDF]
The complete genome sequences of a porcine circovirus type 1 (PCV1) strain isolated in 1990 and one isolated in 2011 were obtained and compared to the sequences of other available PCV1 isolates.
Baylis +7 more
core +1 more source
Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2 [PDF]
Background The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum ...
Shi-Qi Sun +6 more
core +2 more sources
First construction of infectious clone for newly emerging mutation porcine circovirus type 2 (PCV2) followed by comparison with PCV2a and PCV2b genotypes in biological characteristics in vitro [PDF]
Background Porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome (PMWS), is a serious economic problem in the swine industry.
Long J Guo +5 more
core +2 more sources
Droplet Digital CRISPR for Nucleic Acid Detection
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang +4 more
wiley +1 more source
Point-of-service diagnostic technology for detection of swine viral diseases
A research project is underway aiming to develop a field diagnostic tool for six important viruses of the pig sector, namely: African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV ...
Nannucci Lapo +5 more
doaj +1 more source
A novel kirkovirus may be associated with equine gastrointestinal disease
Abstract Background Many cases of equine enterocolitis are suspected to be infectious in nature, but no pathogen is identified in many cases. Objectives Perform next‐generation sequencing on faeces collected from cases of equine enterocolitis for the presence of novel viruses and determine if an identified novel virus is associated with cases of equine
Lillian M. B. Haywood +3 more
wiley +1 more source
Porcine circovirus: Transcription and DNA replication
This review summarizes the molecular studies pertaining to porcine circovirus (PCV) transcription and DNA replication. The genome of PCV is circular, single-stranded DNA and contains 1759-1768 nucleotides. Both the genome-strand (packaged in the virus particle) and the complementary-strand (synthesized in the new host) encode viral proteins.
openaire +2 more sources
Pathogenesis and Transmission of a Reassorted H1 Influenza A Virus Detected in North American Swine
ABSTRACT Background The USDA influenza A virus in swine national surveillance plan identified an increase in the detection frequency of a group of swine 1A.1.1.3 hemagglutinin (HA) clade viruses. This change was associated with reassortment events that resulted in new neuraminidase (NA) gene pairings.
Débora B. Goulart +8 more
wiley +1 more source
Histopathological investigation in porcine infected with torque teno sus virus type 2 by inoculation [PDF]
Background Porcine torque teno sus virus (TTSuV) is a small icosahedral and non-enveloped virus which contains a single-stranded (ssDNA), circular and negative DNA genome and infects mainly vertebrates and is currently classified into the 'floating ...
Miao Mei +8 more
core +2 more sources
First Molecular Detection of Torque Teno Canis Virus in Apparently Healthy Dogs in Southern Italy
•Torque teno canis virus has been detected in dogs from southern Italy. •Detection of TTCaV DNA was more frequent in dogs with an altered faecal score, mixed‐breed and belonging to kennels. ABSTRACT Background Torque teno viruses are viruses with unclear pathogenic potential, as conflicting evidence has classified this family of viruses as harmless and
Gianmarco Ferrara +3 more
wiley +1 more source

