DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate. [PDF]
Viroids are single‐stranded circular noncoding RNAs that mainly infect crops. Upon infection, nuclear‐replicating viroids engage host DNA‐dependent RNA polymerase II for RNA‐templated transcription, which is facilitated by a host protein TFIIIA‐7ZF.
Wang Y, Ma J, Hao J, Liu B, Wang Y.
europepmc +3 more sources
Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and Nicotiana benthamiana [PDF]
Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids.
Ying Wei Khoo +8 more
doaj +3 more sources
Scion on a stock producing siRNAs of potato spindle tuber viroid (PSTVd) attenuates accumulation of the viroid. [PDF]
Plants can attenuate the replication of plant viruses and viroids by RNA silencing induced by virus and viroid infection. In higher plants, silencing signals such as small interfering RNAs (siRNAs) produced by RNA silencing can be transported ...
Atsushi Kasai, Teruo Sano, Takeo Harada
doaj +4 more sources
Tolerance Even to Lethal Strain of Potato Spindle Tuber Viroid Found in Wild Tomato Species Can Be Introduced by Crossing. [PDF]
To date, natural resistance or tolerance, which can be introduced into crops by crossing, to potato spindle tuber viroid (PSTVd) has not been reported.
Naoi T, Hataya T.
europepmc +4 more sources
RNA three-dimensional structure drives the sequence organization of potato spindle tuber viroid quasispecies. [PDF]
RNA viruses and viroids exist and evolve as quasispecies due to error-prone replication. Quasispecies consist of a few dominant master sequences alongside numerous variants that contribute to genetic diversity.
Jian Wu +5 more
doaj +3 more sources
Commodity risk assessment of Petunia spp. and Calibrachoa spp. unrooted cuttings from Uganda [PDF]
The European Commission requested the EFSA Panel on Plant Health to evaluate the likelihood of pest freedom at entry in the EU, including both regulated and non‐regulated pests, potentially associated with unrooted cuttings of the genera Petunia and ...
EFSA Panel on Plant Health (PLH) +34 more
doaj +3 more sources
Insights Into Potato Spindle Tuber Viroid Quasi-Species From Infection to Disease [PDF]
Viroids are non-coding RNA plant pathogens that are characterized by their possession of a high mutation level. Although the sequence heterogeneity in viroid infected plants is well understood, shifts in viroid population dynamics due to mutations over ...
Charith Raj Adkar-Purushothama +3 more
doaj +3 more sources
Evidence Supporting That RNA Polymerase II Catalyzes De Novo Transcription Using Potato Spindle Tuber Viroid Circular RNA Templates. [PDF]
Transcription is a fundamental process that mediates the interplay between genetic information and phenotype. Emerging evidence indicates that RNA polymerase II (Pol II) can catalyze transcription using both DNA and RNA templates.
Dissanayaka Mudiyanselage SD, Wang Y.
europepmc +4 more sources
Analysis of small RNA production patterns among the two potato spindle tuber viroid variants in tomato plants [PDF]
In order to analyze the production of small RNA (sRNA) by viroids upon infecting the plants, the tomato plants (Solanum lycopersicum cultivar Rutgers) were inoculated with the variants of Potato spindle tuber viroid (PSTVd).
Charith Raj Adkar-Purushothama +2 more
doaj +3 more sources
Time-Resolved Observation of the Destination of Microinjected Potato Spindle Tuber Viroid (PSTVd) in the Abaxial Leaf Epidermal Cells of Nicotiana benthamiana [PDF]
Viroids are single-stranded noncoding RNA molecules of 250–400 nucleotides that cause plant diseases. One of the two families of viroids is Pospiviroidae, the members of which replicate in the nuclei of host cells.
Hyesu Seo, Ying Wang, Woong June Park
doaj +4 more sources

