Results 201 to 210 of about 3,953 (249)
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Virology, 1975
Abstract A method has been devised to isolated cell nuclei from uninfected or potato spindle tuber viroid (PSTV)-infected tomato leaves. Properties of the RNA-synthesizing system in nuclei purified from both sources were characterized and compared. Purified nuclei from uninfected and infected leaves have the ability to incorporate [ 3 H]UMP from [ 3 ...
T. Takahashi, Theodor O. Diener
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Abstract A method has been devised to isolated cell nuclei from uninfected or potato spindle tuber viroid (PSTV)-infected tomato leaves. Properties of the RNA-synthesizing system in nuclei purified from both sources were characterized and compared. Purified nuclei from uninfected and infected leaves have the ability to incorporate [ 3 H]UMP from [ 3 ...
T. Takahashi, Theodor O. Diener
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Nucleotide sequence and secondary structure of potato spindle tuber viroid
Nature, 1978The viroid of the potato spindle tuber disease (PSTV) is a covalently closed ring of 359 ribonucleotides. As a result of intramolecular base pairing, a serial arrangement of double-helical sections and internal loops form a unique rod-like secondary structure.
Manfred Raba +6 more
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Plant Disease
Potato (Solanum tuberosum), as an internationally traded, global crop, is exposed to a wide range of diseases that cause economic losses. Economically important pathogens include oomycetes, fungi, viruses, bacteria, and viroids.
Mercedes Ames +4 more
semanticscholar +1 more source
Potato (Solanum tuberosum), as an internationally traded, global crop, is exposed to a wide range of diseases that cause economic losses. Economically important pathogens include oomycetes, fungi, viruses, bacteria, and viroids.
Mercedes Ames +4 more
semanticscholar +1 more source
Journal of Phytopathology
In recent years, Potato spindle tuber viroid (PSTVd), a member of the genus Pospiviroid, has been increasingly detected in a wide range of vegetatively propagated and ornamental plant species.
Z. K. Sial +9 more
semanticscholar +1 more source
In recent years, Potato spindle tuber viroid (PSTVd), a member of the genus Pospiviroid, has been increasingly detected in a wide range of vegetatively propagated and ornamental plant species.
Z. K. Sial +9 more
semanticscholar +1 more source
The distribution of potato spindle tuber viroid in potato plants and tubers1
EPPO Bulletin, 1987Potato spindle tuber viroid (PSTV) in potato plants was investigated by ‘return’ gel electrophoresis. The experiments were carried out under quarantine conditions in the greenhouse with primarily and secondarily infected plants. The PSTV content in different plant parts was estimated by the intensity of the viroid band in polyacrylamide gel.
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Virology, 1971
Abstract By coelectrophoresis of formylated tobacco ringspot satellite virus RNA (SATRNA), formylated transfer RNA, formylated 5 S ribosomal RNA, and formylated carnation mottle virus RNA in polyacrylamide gels, the molecular weight of SATRNA was determined to be between 7.7 × 10 4 and 8.4 × 10 4 . Gel electrophoresis of purified, formylated potato
D. R. Smith, Theodor O. Diener
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Abstract By coelectrophoresis of formylated tobacco ringspot satellite virus RNA (SATRNA), formylated transfer RNA, formylated 5 S ribosomal RNA, and formylated carnation mottle virus RNA in polyacrylamide gels, the molecular weight of SATRNA was determined to be between 7.7 × 10 4 and 8.4 × 10 4 . Gel electrophoresis of purified, formylated potato
D. R. Smith, Theodor O. Diener
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Efficacy of five disinfectants against Potato spindle tuber viroid
Crop Protection, 2015Viroids are RNA molecules which are harmful to numerous cultivated plant species and considered, for some of them, as quarantine organisms. Because these pathogens are readily transmitted by contact, effective disinfection measures to clean the cultivating tools, machineries or facilities have to be found and approved.
T. Olivier +7 more
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Detection of potato spindle tuber viroid (PSTV) in dormant potato tubers by concatameric cDNA probe
Journal of Virological Methods, 1990The 32P-labelled concatameric insert cut out from a plasmid pSPAv6.2(+), containing 6.2 copies of a full-length PSTV, was used to detect PSTV in dormant potato tubers by dot-blot hybridisation assay. The concatameric insert probe was 4 times more sensitive than the monomeric one. This allowed the detection of 0.5 pg of viroid RNA. The sensitivity makes
J. Skrzeczkowski +3 more
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Virology, 1974
Abstract The ability of potato spindle tuber viroid (PSTV) RNA to act as a messenger RNA for protein synthesis has been investigated in several cell-free protein synthesizing systems. Extracts from wheat embryo, commercial wheat germ, Escherichia coli and Bacillus stearothermophilus , which are known to translate RNAs from viruses that do not ...
J.W. Davies +5 more
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Abstract The ability of potato spindle tuber viroid (PSTV) RNA to act as a messenger RNA for protein synthesis has been investigated in several cell-free protein synthesizing systems. Extracts from wheat embryo, commercial wheat germ, Escherichia coli and Bacillus stearothermophilus , which are known to translate RNAs from viruses that do not ...
J.W. Davies +5 more
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Inhibition of potato spindle tuber viroid (PSTVd) infection with DNA oligonucleotides
Biochimie, 1993The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 of PSTVd was hybridized with PSTVd ...
L Arnold +6 more
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