The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests [PDF]
An assay was developed to detect the potato spindle tuber viroid (PSTVd), a dangerous plant pathogen that causes crop damage resulting in economic losses in the potato agriculture sector.
Aleksandr V. Ivanov+4 more
doaj +3 more sources
Suppression of RNA-dependent RNA polymerase 6 in tomatoes allows potato spindle tuber viroid to invade basal part but not apical part including pluripotent stem cells of shoot apical meristem. [PDF]
RNA-dependent RNA polymerase 6 (RDR6) is one of the key factors in plant defense responses and suppresses virus or viroid invasion into shoot apical meristem (SAM) in Nicotiana benthamiana.
Takashi Naoi+7 more
doaj +3 more sources
Allelic RNA Motifs in Regulating Systemic Trafficking of Potato Spindle Tuber Viroid [PDF]
Intercellular RNA trafficking has been shown as a widely-existing phenomenon that has significant functions in many aspects of biology. Viroids, circular noncoding RNAs that cause plant diseases, have been a model to dissect the role of RNA structural ...
Ryuta Takeda+4 more
doaj +4 more sources
Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo. [PDF]
Research in the laboratory of R. F. is currently funded by grant BFU2011-28443 from the Ministerio de Economia y Competitividad (MINECO, Spain). S.M. has been supported by a fellowship and a pre-doctoral contract from MINECO.
Minoia S+6 more
europepmc +3 more sources
Tolerance Even to Lethal Strain of Potato Spindle Tuber Viroid Found in Wild Tomato Species Can Be Introduced by Crossing. [PDF]
To date, natural resistance or tolerance, which can be introduced into crops by crossing, to potato spindle tuber viroid (PSTVd) has not been reported.
Naoi T, Hataya T.
europepmc +2 more sources
Interdependence of Pathogenicity and Replicability with Potato Spindle Tuber Viroid
After the unexpected appearance of lethal symptoms on tomato plants infected with the PSTVd strain Intermediate Di, viroids were isolated and sequenced. It was found that a new strain, named RG 1, had been generated spontaneously in our greenhouse.
Detlev Riesner+5 more
openaire +4 more sources
Several viroids in the genus Pospiviroid can infect tomato (Solanum lycopersicum) and cause severe diseases, posing a serious threat to tomato production.
Yu-hong ZHANG+4 more
doaj +2 more sources
Genetic variability of potato spindle tuber viroid RNA replicon.
The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms.
Gora-Sochacka, A.+2 more
+10 more sources
Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs [PDF]
As a consequence of two unique physical properties, small size and circularity, viroid RNAs do not code for proteins and thus depend on RNA sequence/structural motifs for interacting with host proteins that mediate their invasion, replication, spread ...
Ricardo eFlores+4 more
doaj +2 more sources
Suppression of RNA-Dependent RNA Polymerase 6 Favors the Accumulation of Potato Spindle Tuber Viroid in Nicotiana Benthamiana [PDF]
To date, two plant genes encoding RNA-dependent RNA polymerases (RdRs) that play major roles in the defense against RNA viruses have been identified: (i) RdR1, which is responsible for the viral small RNAs (vsRNAs) found in virus-infected plants, and ...
Charith Raj Adkar-Purushothama+1 more
doaj +2 more sources