Results 51 to 60 of about 2,571 (207)

Development and validation of a real-time RT-PCR test for screening pepper and tomato seed lots for the presence of pospiviroids.

open access: yesPLoS ONE, 2020
Potato spindle tuber viroid and other pospiviroids can cause serious diseases in potato and tomato crops. Consequently, pospiviroids are regulated in several countries. Since seed transmission is considered as a pathway for the introduction and spread of
Marleen Botermans   +11 more
doaj   +1 more source

Dynamic co-expression modular network analysis of bHLH transcription factors regulation in the potato spindle tuber viroid-tomato pathosystem

open access: yesbioRxiv, 2023
Viroids, minimalist plant pathogens, present significant threats to crops by causing severe diseases. The use of high-throughput sequencing technologies for analyzing the transcriptomes of viroid-infected host plants has yielded informative information ...
Katia Aviña-Padilla   +4 more
semanticscholar   +1 more source

RNA intermediates in potato spindle tuber viroid replication [PDF]

open access: yesProceedings of the National Academy of Sciences, 1982
Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA-RNA hybridization techniques.
R A, Owens, T O, Diener
openaire   +2 more sources

Precisely Monomeric Linear RNAs of Viroids Belonging to Pospiviroid and Hostuviroid Genera Are Infectious Regardless of Transcription Initiation Site and 5′-Terminal Structure

open access: yesCells, 2021
Infectious dimeric RNA transcripts are a powerful tool for reverse genetic analyses in viroid studies. However, the construction of dimeric cDNA clones is laborious and time consuming, especially in mutational analyses by in vitro mutagenesis.
Tatsuji Hataya, Takashi Naoi
doaj   +1 more source

Construction of infectious potato spindle tuber viroid cDNA clones [PDF]

open access: yesNucleic Acids Research, 1983
Contiguous restriction fragments from two cloned partial-length potato spindle tuber viroid (PSTV) cDNAs were used to construct recombinant DNAs containing full-length monomeric and dimeric PSTV cDNA. When five different PSTV cDNA plasmids and RNA isolated from E.
D E, Cress, M C, Kiefer, R A, Owens
openaire   +2 more sources

Detection of Peach latent mosaic viroid by PCR

open access: yesPlant Protection Science, 2002
Peach latent mosaic viroid (PLMVd) is widespread in peach all over the world. It has never been reported from the Czech Republic. That is why we adapted specific and sensitive method for its detection, PCR, to be able to prove its possible occurrence and
P. Ryšánek, M. Zouhar, M. Hassan
doaj   +1 more source

Cell‐to‐cell movement of potato spindle tuber viroid [PDF]

open access: yesThe Plant Journal, 1997
SummaryViroids are non‐translatable, autonomously replicating circular RNAs that infect only plants. An important component of the viroid infection process is cell‐to‐cell movement; however, there is virtually no information available about the pathways and mechanisms of this process.
B, Ding, M O, Kwon, R, Hammond, R, Owens
openaire   +2 more sources

Vertical and Horizontal Transmission of Pospiviroids

open access: yesViruses, 2018
Viroids are highly structured, single-stranded, non-protein-coding circular RNA pathogens. Some viroids are vertically transmitted through both viroid-infected ovule and pollen.
Yosuke Matsushita   +2 more
doaj   +1 more source

Home - About - Disclaimer - Privacy