Results 181 to 190 of about 4,765 (208)
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Modulation of RNA polymerase specificity by ppGpp
Molecular and General Genetics MGG, 1976ppGpp alters the initiation specificity of RNA polymerase holoenzyme in vitro in a direction which mimics the stringent response in vivo. The transition temperature for opening rRNA promoters is increased by the nucleotide, that for opening phi80 promoters is unaffected.
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A gene involved in the metabolic control of ppGpp synthesis
Molecular and General Genetics MGG, 1978A genetic locus has been identified which controls the basal synthesis of ppGpp in growing E. coli. Cells carrying a recessive allele of the relX gene have a very low concentration of ppGpp during balanced growth, and fail to accumulate ppGpp in response to carbon/energy source downshift.
C C, Pao, J, Gallant
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1980
Faced with a shortage of nutrient source, bacteria rapidly adjust their metabolism and adapt to a more frugal life style. Of the doubtless many overlapping and hierarchical controls required for such an adjustment, a simple mechanism mediated by the peculiar nucleotide guanosine 5′-diphosphate,3′-diphosphate (ppGpp) will be reviewed and its metabolism ...
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Faced with a shortage of nutrient source, bacteria rapidly adjust their metabolism and adapt to a more frugal life style. Of the doubtless many overlapping and hierarchical controls required for such an adjustment, a simple mechanism mediated by the peculiar nucleotide guanosine 5′-diphosphate,3′-diphosphate (ppGpp) will be reviewed and its metabolism ...
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Lack of accumulation of ppGpp in sea urchin embryos
Developmental Biology, 1976Abstract Sea urchin eggs and embryos of a variety of stages were labeled with [ 32 P]orthophosphate. We have been unable to detect the accumulation of guanosine tetraphosphate (Magic Spot I) at any stage. We point out a potential artifact in screening cells for the synthesis of Magic Spot. I.
B, Brandhorst, D, Fromson
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The mechanism of action of ppGpp on rRNA synthesis in vitro
Cell, 1976We have studied the mechanism of the specific inhibition of ribosomal RNA synthesis by ppGpp in a purified system using as templates E. coli DNA and DNA from lambdad5ilv, which carries a rRNA cistron from E. coli. Ribosomal RNA synthesis, as well as its inhibition by ppGpp, are critically salt-dependent. Of a number of guanosine phosphates tested, only
van Ooyen, A J +2 more
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Kinetic suppression of translational errors by (p)ppGpp
Molecular and General Genetics MGG, 1982The effect of (p)ppGpp on the accuracy of translation was studied in vitro using a poly(U)-programmed poly(Phe)-synthesizing system operating at incorporation rates and missense error frequencies close to the values obtained in vivo. Simulation of a relaxed phenotype in vitro was accomplished by limitation of the cognate aminoacyl-tRNA synthetase ...
E. Gerhart +3 more
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Revisiting the stringent response, ppGpp and starvation signaling
Current Opinion in Microbiology, 2001Microbial adaptation to environmental stress plays an important role in survival. It is necessary to understand the mechanisms underlying the survival of microbes under stress, as they may eventually aid in the successful control of the growth and persistence of these organisms.
Chatterji, Dipankar, Ojha, Anil Kumar
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Microbiology and Molecular Biology Reviews
SUMMARY Bacteria are constantly exposed to changing environmental conditions, and to survive they need to adapt quickly, adjusting their gene expression and metabolism to make the most of the resources available. One of the mechanisms involved is the stringent response, characterized by production of specific guanosine derivatives ...
Katarzyna Potrykus +3 more
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SUMMARY Bacteria are constantly exposed to changing environmental conditions, and to survive they need to adapt quickly, adjusting their gene expression and metabolism to make the most of the resources available. One of the mechanisms involved is the stringent response, characterized by production of specific guanosine derivatives ...
Katarzyna Potrykus +3 more
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A tRNATyr promoter with an altered in vitro response to ppGpp
Journal of Molecular Biology, 1980A synthetic alteration in the promoter sequence of the tRNA Tyr gene from (Ryan et al. , 1979) alters the in vitro regulation of the promoter by ppGpp such that synthesis of RNA from the mutant promoter responds to increasing concentrations of ppGpp in a reciprocal manner to that from the wild-type promoter.
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ppGpp: stringent response and survival.
Journal of microbiology (Seoul, Korea), 2006Adaptation to any undesirable change in the environment dictates the survivability of many microorganisms, with such changes generating a quick and suitable response, which guides the physiology of bacteria. During nutritional deprivation, bacteria show a stringent response, as characterized by the accumulation of (p)ppGpp, resulting in the repression ...
Jain, Vikas +2 more
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