Results 111 to 120 of about 14,388,284 (282)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Asteroseismology of intermediate mass stars : pre-main sequence evolution [PDF]

, 2007
xiv, 130 leaves : ill. ; 29 cm.Includes abstract.Includes bibliographical references (leaves 126-130).We investigate in detail the potential of asteroseismology in exploring the structure of intermediate mass (1.6 ≤ M⊙ ≤ 5.0) pre-main ...
Tanner, Joel
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

X-Ray Evolution of Young Stars: Early Dimming and Coronal Softening in Solar-mass Stars with Implications for Planetary Atmospheres

The Astrophysical Journal
X-ray and ultraviolet (XUV) emission from young stars plays a critical role in shaping the evolution of planetary atmospheres and the conditions for habitability.
Konstantin V. Getman, Eric D. Feigelson, Vladimir S. Airapetian, Gordon P. Garmire   +3 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Orbital motion and dynamical mass of the complex periodic variable binary system 2MASS J05082729–2101444

Astronomy & Astrophysics
Aims. This study focuses on the low-mass binary 2MASS J05082729-2101444 (2M0508-21), one of the few known radio-loud members of the complex periodic variable sample. Our aim is to use very long baseline interferometry to constrain the orbit. Methods.
Curiel S., Ortiz-León G. N., Béjar V. J. S., Viganò D., Girart J. M., Kaur S., Shan Y., Murgas F., Zechmeister M., Amado P. J., Caballero J. A., Henning Th., Ilin E., Montes D., Morales J. C., Morata Ò., Pérez-Torres M., Quirrenbach A., Reiners A., Ribas I., Sánchez-Monge Á., Schweitzer A., Vico Linares J. I., Zapatero Osorio M. R.   +23 more
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Solar-like Oscillations in Accreting Pre-main-sequence Stars: Insights and Prospects

The Astrophysical Journal
We present theoretical predictions for solar-like oscillators in the pre-main-sequence (pre-MS) phase of stellar evolution. Our pre-MS models start from a stellar seed of 0.01 solar masses that gains mass through accretion, offering an alternative ...
Johannes Jørgensen, Konstanze Zwintz, Ebraheem Farag, Eduard I. Vorobyov, Thomas Steindl   +4 more
doaj   +1 more source