Results 31 to 40 of about 510,232 (259)

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Nature Communications, 2022
Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li, Michael R. Wasserman, Olga Yurieva, Lu Bai, Michael E. O’Donnell, Shixin Liu   +5 more
doaj   +1 more source

MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion

eLife, 2018
DNA replication transforms cohesin rings dynamically associated with chromatin into the cohesive form to establish sister-chromatid cohesion. Here, we show that, in human cells, cohesin loading onto chromosomes during early S phase requires the ...
Ge Zheng, Mohammed Kanchwala, Chao Xing, Hongtao Yu   +3 more
doaj   +1 more source

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Cell Reports, 2020
Summary: Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle.
Aftab Amin, Rentian Wu, Man Hei Cheung, John F. Scott, Ziyi Wang, Zijing Zhou, Changdong Liu, Guang Zhu, Chris Kong-Chu Wong, Zhiling Yu, Chun Liang   +10 more
doaj   +1 more source

Preferential re-replication of Drosophila heterochromatin in the absence of geminin. [PDF]

PLoS Genetics, 2010
To ensure genomic integrity, the genome must be duplicated exactly once per cell cycle. Disruption of replication licensing mechanisms may lead to re-replication and genomic instability.
Queying Ding, David M MacAlpine
doaj   +1 more source

Emerging players in the initiation of eukaryotic DNA replication

Cell Division, 2012
Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase; transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication ...
Shen Zhen, Prasanth Supriya G
doaj   +1 more source

The non-canonical role of Aurora-A in DNA replication

Frontiers in Oncology, 2015
Aurora-A is a well-known mitotic kinase that regulates mitotic entry, spindle formation, and chromosome maturation as a canonical role. During mitosis, Aurora-A protein is stabilized by its phosphorylation at Ser51 via blocking anaphase promoting complex/
Takaaki eTsunematsu, Rieko eArakaki, Akiko eYamada, Naozumi eIshimaru, Yasusei eKudo   +4 more
doaj   +1 more source

Genome-wide hierarchy of replication origin usage in Saccharomyces cerevisiae. [PDF]

PLoS Genetics, 2006
Replication origins in a genome are inherently different in their base sequence and in their response to temporal and cell cycle regulation signals for DNA replication.
Justin J Donato, Shau Chee C Chung, Bik K Tye   +2 more
doaj   +1 more source

LSD1 is required for euchromatic origin firing and replication timing

Signal Transduction and Targeted Therapy, 2022
The chromatin-based rule governing the selection and activation of replication origins remains to be elucidated. It is believed that DNA replication initiates from open chromatin domains; thus, replication origins reside in open and active chromatin ...
Yue Wang, Yunchao Huang, Edith Cheng, Xinhua Liu, Yu Zhang, Jianguo Yang, Jordan T. F. Young, Grant W. Brown, Xiaohan Yang, Yongfeng Shang   +9 more
doaj   +1 more source

Cryo-EM structure of human hexameric MCM2-7 complex

iScience, 2022
Summary: The central step in the initiation of eukaryotic DNA replication is the loading of the minichromosome maintenance 2–7 (MCM2-7) complex, the core of the replicative DNA helicase, onto chromatin at replication origin. Here, we reported the cryo-EM
Naining Xu, Qingpeng Lin, Honglei Tian, Changdong Liu, Peiyi Wang, Ching Monica Suen, Hongyu Yang, Ye Xiang, Guang Zhu   +8 more
doaj   +1 more source

Cdt1 degradation to prevent DNA re-replication: conserved and non-conserved pathways

Cell Division, 2007
In eukaryotes, DNA replication is strictly regulated so that it occurs only once per cell cycle. The mechanisms that prevent excessive DNA replication are focused on preventing replication origins from being reused within the same cell cycle.
Kim Youngjo, Kipreos Edward T
doaj   +1 more source