Results 31 to 40 of about 702,020 (309)
Bioinformatical dissection of fission yeast DNA replication origins [PDF]
Open Biology, 2020 Replication origins in eukaryotes form a base for assembly of the pre-replication complex (pre-RC), thereby serving as an initiation site of DNA replication. Characteristics of replication origin vary among species.
Koji Masuda +3 more
doaj +1 more source
The human pre-replication complex is an open complex
Cell, 2023 In eukaryotes, DNA replication initiation requires assembly and activation of the minichromosome maintenance (MCM) 2-7 double hexamer (DH) to melt origin DNA strands. However, the mechanism for this initial melting is unknown. Here, we report a 2.59-Å cryo-electron microscopy structure of the human MCM-DH (hMCM-DH), also known as the pre-replication ...
Li, Jian +15 more
openaire +4 more sources
Transient dsDNA breaks during pre-replication complex assembly [PDF]
Nucleic Acids Research, 2009 Initiation of DNA replication involves the ordered assembly of the multi-protein pre-replicative complex (pre-RC) during G(1) phase. Previously, DNA topoisomerase II (topo II) was shown to associate with the DNA replication origin located in the lamin B2 gene locus in a cell-cycle-modulated manner.
Rampakakis, Emmanouil +1 more
openaire +2 more sources
A Role of hIPI3 in DNA Replication Licensing in Human Cells. [PDF]
PLoS ONE, 2016 The yeast Ipi3p is required for DNA replication and cell viability in Sacharomyces cerevisiae. It is an essential component of the Rix1 complex (Rix1p/Ipi2p-Ipi1p-Ipi3p) that is required for the processing of 35S pre-rRNA in pre-60S ribosomal particles ...
Yining Huang +7 more
doaj +1 more source
Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.
PLoS Genetics, 2021 The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of ...
Eric J Foss +6 more
doaj +1 more source
The role of pre‐replicative complex (pre‐RC) components in oncogenesis [PDF]
The FASEB Journal, 2007 Normal DNA replication is stringently regulated to ensure a timely occurrence no more than once per cell cycle. Abrogation of the exquisite control mechanisms that maintain this process results in detrimental gains and losses of genomic DNA commonly seen in cancer and developmental defects. Replication initiation proteins, known as
Eric, Lau +4 more
openaire +4 more sources
Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
doaj +1 more source
The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]
, 2012 Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Anton Gartner +56 more
core +4 more sources
MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion
eLife, 2018 DNA replication transforms cohesin rings dynamically associated with chromatin into the cohesive form to establish sister-chromatid cohesion. Here, we show that, in human cells, cohesin loading onto chromosomes during early S phase requires the ...
Ge Zheng +3 more
doaj +1 more source