Results 41 to 50 of about 510,232 (259)

Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone

Nucleic Acids Research, 2010
Genome-scale mapping of pre-replication complex proteins has not been reported in mammalian cells. Poor enrichment of these proteins at specific sites may be due to dispersed binding, poor epitope availability or cell cycle stage-specific binding.
Yoav Lubelsky, Takayo Sasaki, Marjorie A. Kuipers, I. Lucas, M. L. Le Beau, S. Carignon, M. Debatisse, Joseph A. Prinz, J. Dennis, D. Gilbert   +9 more
semanticscholar   +1 more source

NFIB facilitates replication licensing by acting as a genome organizer

Nature Communications, 2023
The chromatin-based rule governing the selection and activation of replication origins in metazoans remains to be investigated. Here we report that NFIB, a member of Nuclear Factor I (NFI) family that was initially purified in host cells to promote ...
Wenting Zhang, Yue Wang, Yongjie Liu, Cuifang Liu, Yizhou Wang, Lin He, Xiao Cheng, Yani Peng, Lu Xia, Xiaodi Wu, Jiajing Wu, Yu Zhang, Luyang Sun, Ping Chen, Guohong Li, Qiang Tu, Jing Liang, Yongfeng Shang   +17 more
doaj   +1 more source

The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.

PLoS Pathogens, 2016
The origin recognition complex (ORC) coordinates a series of events that lead to initiation of DNA strand duplication. As a nuclear double stranded DNA plasmid, the papillomavirus (PV) genome resembles a mini-chromosome in infected cells. To initiate its
Marsha DeSmet, Sriramana Kanginakudru, Anne Rietz, Wai-Hong Wu, Richard Roden, Elliot J Androphy   +5 more
doaj   +1 more source

Minichromosome Maintenance Proteins: From DNA Replication to the DNA Damage Response

Cells
The DNA replication machinery is highly conserved from bacteria to eukaryotic cells. Faithful DNA replication is vital for cells to transmit accurate genetic information to the next generation. However, both internal and external DNA damages threaten the
Agnes Malysa, Xiaohong Mary Zhang, Gerold Bepler   +2 more
doaj   +1 more source

Identification of ORC1/CDC6-interacting factors in Trypanosoma brucei reveals critical features of origin recognition complex architecture.

PLoS ONE, 2012
DNA replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with ...
Calvin Tiengwe, Lucio Marcello, Helen Farr, Catarina Gadelha, Richard Burchmore, J David Barry, Stephen D Bell, Richard McCulloch   +7 more
doaj   +1 more source

LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities.

PLoS Pathogens, 2023
LGP2 is a RIG-I-like receptor (RLR) known to bind and recognize the intermediate double-stranded RNA (dsRNA) during virus infection and to induce type-I interferon (IFN)-related antiviral innate immune responses.
Zhongyuan Tan, Jiqin Wu, Li Huang, Ting Wang, Zhenhua Zheng, Jianhui Zhang, Xianliang Ke, Yuan Zhang, Yan Liu, Hanzhong Wang, Jianping Tao, Peng Gong   +11 more
doaj   +1 more source

Over expression of minichromosome maintenance genes is clinically correlated to cervical carcinogenesis. [PDF]

PLoS ONE, 2013
Minichromosome Maintenance (MCM) proteins play important roles in cell cycle progression by mediating DNA replication initiation and elongation. Among 10 MCM homologues MCM 2-7 form a hexamer and assemble to the pre-replication complex acting as ...
Mitali Das, Shyam Babu Prasad, Suresh Singh Yadav, H B Govardhan, Lakshmi Kant Pandey, Sunita Singh, Satyajit Pradhan, Gopeshwar Narayan   +7 more
doaj   +1 more source

A microhomology-mediated break-induced replication model for the origin of human copy number variation. [PDF]

PLoS Genetics, 2009
Chromosome structural changes with nonrecurrent endpoints associated with genomic disorders offer windows into the mechanism of origin of copy number variation (CNV).
P J Hastings, Grzegorz Ira, James R Lupski   +2 more
doaj   +1 more source

The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans

Biology, 2023
Simple Summary The origin recognition complex (ORC) selects sites for replication initiation by recruiting a pair of hexameric minichromosome maintenance (MCM) complexes to replication origins where the pre-replication complex (Pre-RC) is assembled, and ...
B. Tye, Y. Zhai
semanticscholar   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source