Results 41 to 50 of about 702,020 (309)

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Cell Reports, 2020
Summary: Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle.
Aftab Amin, Rentian Wu, Man Hei Cheung, John F. Scott, Ziyi Wang, Zijing Zhou, Changdong Liu, Guang Zhu, Chris Kong-Chu Wong, Zhiling Yu, Chun Liang   +10 more
doaj   +1 more source

Preferential re-replication of Drosophila heterochromatin in the absence of geminin. [PDF]

PLoS Genetics, 2010
To ensure genomic integrity, the genome must be duplicated exactly once per cell cycle. Disruption of replication licensing mechanisms may lead to re-replication and genomic instability.
Queying Ding, David M MacAlpine
doaj   +1 more source

Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA [PDF]

, 2013
In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA.
A Costa, A Fernández-Cid, Alberto Riera, Alejandra Fernández-Cid, AS Brewster, AY Lyubimov, B Stillman, BA Kelch, BI Khayrutdinov, Bruce Stillman, C Evrin, C Evrin, C Liu, C Santocanale, C Speck, C Speck, Cecile Evrin, Christian Speck, D Remus, D Remus, D Suck, E Arias-Palomo, EF Pettersen, EL Dueber, G Tang, GC Lander, GD Bowman, GD Pintilie, H Li, H Li, Hironori Kawakami, Huilin Li, I Ilves, IN Chesnokov, J Frigola, J Liu, J Sun, JC Randell, Jingchuan Sun, JL Bowers, L Zou, M Gaudier, M O'Donnell, MG Clarey, MG Clarey, ML Baker, ML Bochman, ML Bochman, ML Bochman, PW Lau, R Henderson, RC Heller, RD Klemm, RJ Fletcher, S Chen, S Donovan, S Onesti, S Waga, SE Moyer, SL Forsburg, SP Bell, SP Bell, Stefan A Samel, T Miyata, T Pape, Y Ishimi, Y Kawasaki, YH Kang, Z Chen   +68 more
core   +1 more source

Emerging players in the initiation of eukaryotic DNA replication

Cell Division, 2012
Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase; transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication ...
Shen Zhen, Prasanth Supriya G
doaj   +1 more source

Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro [PDF]

, 2010
Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs).
Ainscough, Berezney, Brown, Cardoso, Chevalier, Cook, Coverley, Coverley, den Hollander, Dijkwel, Djeliova, Fujita, Furstenthal, Geng, Geng, Harper, Hozak, Krude, Lowe, Mailand, Mitsui, Moore, Parisi, Radichev, Sherr, Stoeber, Su, Takeda, Tatsumi, Wilmes, Wohlschlegel   +30 more
core   +3 more sources

The non-canonical role of Aurora-A in DNA replication

Frontiers in Oncology, 2015
Aurora-A is a well-known mitotic kinase that regulates mitotic entry, spindle formation, and chromosome maturation as a canonical role. During mitosis, Aurora-A protein is stabilized by its phosphorylation at Ser51 via blocking anaphase promoting complex/
Takaaki eTsunematsu, Rieko eArakaki, Akiko eYamada, Naozumi eIshimaru, Yasusei eKudo   +4 more
doaj   +1 more source

Pre-replicative complex assembly with purified proteins

Methods, 2012
Licensing of origins of eukaryotic DNA replication involves the loading of six minichromosome maintenance proteins (Mcm2-7) into pre-replicative complexes (pre-RCs). The assembly of the pre-RC is restricted to G1 phase of the cell cycle, which is crucial to ensure once per cell cycle DNA replication.
Amina Mehanna, John F.X. Diffley
openaire   +2 more sources

Genome-wide hierarchy of replication origin usage in Saccharomyces cerevisiae. [PDF]

PLoS Genetics, 2006
Replication origins in a genome are inherently different in their base sequence and in their response to temporal and cell cycle regulation signals for DNA replication.
Justin J Donato, Shau Chee C Chung, Bik K Tye   +2 more
doaj   +1 more source

LSD1 is required for euchromatic origin firing and replication timing

Signal Transduction and Targeted Therapy, 2022
The chromatin-based rule governing the selection and activation of replication origins remains to be elucidated. It is believed that DNA replication initiates from open chromatin domains; thus, replication origins reside in open and active chromatin ...
Yue Wang, Yunchao Huang, Edith Cheng, Xinhua Liu, Yu Zhang, Jianguo Yang, Jordan T. F. Young, Grant W. Brown, Xiaohan Yang, Yongfeng Shang   +9 more
doaj   +1 more source

Cryo-EM structure of human hexameric MCM2-7 complex

iScience, 2022
Summary: The central step in the initiation of eukaryotic DNA replication is the loading of the minichromosome maintenance 2–7 (MCM2-7) complex, the core of the replicative DNA helicase, onto chromatin at replication origin. Here, we reported the cryo-EM
Naining Xu, Qingpeng Lin, Honglei Tian, Changdong Liu, Peiyi Wang, Ching Monica Suen, Hongyu Yang, Ye Xiang, Guang Zhu   +8 more
doaj   +1 more source