Results 71 to 80 of about 702,020 (309)

A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations. [PDF]

, 2012
BackgroundEukaryotic cell proliferation involves DNA replication, a tightly regulated process mediated by a multitude of protein factors. In budding yeast, the initiation of replication is facilitated by the heterohexameric origin recognition complex ...
DaSilva, Lance F, Duncker, Bernard P, Gidvani, Rohan D, Ingalls, Brian P, Li, Grace, McConkey, Brendan J, Sudmant, Peter   +6 more
core   +3 more sources

The MedSupport Multilevel Intervention to Enhance Support for Pediatric Medication Adherence: Development and Feasibility Testing

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Introduction We developed MedSupport, a multilevel medication adherence intervention designed to address root barriers to medication adherence. This study sought to explore the feasibility and acceptability of the MedSupport intervention strategies to support a future full‐scale randomized controlled trial.
Elizabeth G. Bouchard, Hital Patel, Paula C. Vincent, Justine Kahn, Kira Bona, Thai Hoa Tran, Elisa M. Rodriguez, Kristopher Attwood, Kara M. Kelly   +8 more
wiley   +1 more source

Stabilisation of half MCM ring by Cdt1 during DNA insertion

Nature Communications, 2021
During pre-Replication Complex, eukaryotic cells load two MCMs into a head-to-head Double Hexamer around duplex DNA (DH). Here the authors preRC assembly assay with purified proteins to reveal insights into S. cerevisiae’s first steps that lead to the DH
Marina Guerrero-Puigdevall, Narcis Fernandez-Fuentes, Jordi Frigola   +2 more
doaj   +1 more source

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells [PDF]

, 2010
Nucleotide excision repair (NER) is the most versatile DNA repair system that deals with the major UV photoproducts in DNA, as well as many other DNA adducts.
Cloney, Ross, Fousteri, Maria I, Japers, Nicolaas G, Lehmann, Alan R, Limsirichaikul, Siripan, Miki, Yoshio, Mullenders., Leon H F, Nakazawa, Yuka, Niimi, Atsuko, Ogi, Tomoo, Overmeer, René M, Takenaka, Katsuya, Volker, Marcel, Yamashita, Shunichi   +13 more
core   +3 more sources

Geminin Inhibits a Late Step in the Formation of Human Pre-replicative Complexes [PDF]

Journal of Biological Chemistry, 2014
The initial step in initiation of eukaryotic DNA replication involves the assembly of pre-replicative complexes (pre-RCs) at origins of replication during the G1 phase of the cell cycle. In metazoans initiation is inhibited by the regulatory factor Geminin.
Wu, Min, Lu, Wenyan, Santos, Ruth E., Frattini, Mark G., Kelly, Thomas J.   +4 more
openaire   +2 more sources

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization [PDF]

, 2013
The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2–7 onto DNA. Helicase loading involves two MCM2–7 hexamers that assemble into a double hexamer around
A. Fernandez-Cid, A. Riera, Bell, Bowers, Bray, C. Evrin, C. Speck, Casas-Terradellas, Chen, Cocker, Coleman, Costa, Donovan, Dueber, Gillespie, Herbig, Holm, J. Zech, Klemm, Krupinski, Laemmli, Liang, Liang, Liu, M. C. Herrera, Mizushima, Neuwald, P. Clarke, Perkins, R. Lurz, Randell, Remus, Remus, Roy, S. Brill, Schepers, Seki, Singleton, Steven, Stoeber, Takara, Tanaka, Watanabe, Weinreich, Wendler, Williams, Ying, Zhang   +47 more
core   +1 more source

Mouse pre-replicative complex proteins colocalise and interact with the centrosome

European Journal of Cell Biology, 2007
Initiation of eukaryotic DNA replication is achieved by the sequential binding of different proteins to origins of DNA replication. Using EGFP-tagged initiator proteins and immunofluorescence techniques we found that most of the ORC and the MCM subunits are localised at centrosomes and are colocalised with the polo-like protein kinase, Plk1.
Andrea, Stuermer, Karsten, Hoehn, Thomas, Faul, Tanja, Auth, Normen, Brand, Margot, Kneissl, Vera, Pütter, Friedrich, Grummt   +7 more
openaire   +2 more sources

The role of fibroblast growth factors in cell and cancer metabolism

FEBS Letters, EarlyView.
Fibroblast growth factor (FGF) signaling regulates crucial signaling cascades that promote cell proliferation, survival, and metabolism. Therefore, FGFs and their receptors are often dysregulated in human diseases, including cancer, to sustain proliferation and rewire metabolism.
Jessica Price, Chiara Francavilla
wiley   +1 more source

The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing

Nature Communications, 2019
DNA replication is initiated at defined genomic sites called origins of replication following ORC pre-replicative complex assembly. Here the authors identify a protein ubiquitylating ORC that is involved in origin activation and may act as a selector of ...
Philippe Coulombe, Joelle Nassar, Isabelle Peiffer, Slavica Stanojcic, Yvon Sterkers, Axel Delamarre, Stéphane Bocquet, Marcel Méchali   +7 more
doaj   +1 more source