By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
FEBS Letters, EarlyView.The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source
A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication.
PLoS Genetics, 2012 Cdc6p is an essential component of the pre-replicative complex (pre-RC), which binds to DNA replication origins to promote initiation of DNA replication. Only once per cell cycle does DNA replication take place.
Amy E Ikui +3 more
doaj +1 more source
eLife, 2021 The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an
Hsiang-Chen Chou +12 more
doaj +1 more source
The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction. [PDF]
, 2018 PINA is a novel ATPase and DNA helicase highly conserved in Archaea, the third domain of life. The PINA from Sulfolobus islandicus (SisPINA) forms a hexameric ring in crystal and solution.
Ahmad, Sohail +9 more
core +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
EEG data and introspective reports from the Libet׳s experiment replication
Data in Brief, 2018 This article provides data from a contemporary replication of Libet׳s experiment. For the methodology, results and discussion of the replication, see the article “Libet׳s Experiment: A Complex Replication” (Dominik et al., 2018).
Tomáš Dominik +5 more
doaj +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation
Nature Communications, 2018 Prereplicative complex (pre-RC) formation during G1 is fundamental for cell replication. Here the authors report a role for cyclin K in regulating pre-RC formation in mammalian cells by affecting cyclin E1 activity.
Tingjun Lei +18 more
doaj +1 more source
Single molecule analysis of replicated DNA reveals the usage of multiple KSHV genome regions for latent replication. [PDF]
PLoS Pathogens, 2011 Kaposi's sarcoma associated herpesvirus (KSHV), an etiologic agent of Kaposi's sarcoma, Body Cavity Based Lymphoma and Multicentric Castleman's Disease, establishes lifelong latency in infected cells.
Subhash C Verma +6 more
doaj +1 more source
Studying complex adaptive systems using molecular classifier systems [PDF]
, 2007 Complex Adaptive Systems (CAS) are dynamical networks of interacting agents occurring in a variety of natural and artificial systems (e.g. cells, societies, stock markets). These complex systems have the ability to adapt, evolve and learn from experience.
Decraene, James +2 more
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