Results 181 to 190 of about 8,556 (220)
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Regulators of Ovarian Preantral Follicle Development
Seminars in Reproductive Medicine, 2015Preantral follicle development has become an increasingly recognized area of study in the last two decades. Factors that regulate the growth survival and differentiation of these small, yet complex structures have been identified. The field of fertility preservation and a need for increased numbers of mature oocytes for stem cell research revealed how ...
Elizabeth A, McGee, Renju S, Raj
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In vitro culture of bovine preantral follicles
Animal Reproduction Science, 2000Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM ...
S, Saha, M, Shimizu, M, Geshi, Y, Izaike
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In vitro culture methods of preantral follicles
Theriogenology, 2016Folliculogenesis is a highly regulated, dynamic process requiring various autocrine, paracrine, and endocrine factors that need to be reproduced in vitro. The mammalian ovary consists of a finite number of follicles at birth, with the majority in a quiescent state, awaiting recruitment.
Lisa J, Green, Ariella, Shikanov
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Xenobiotic effects on ovarian preantral follicles.
Biology of reproduction, 2012Women are born with a finite population of ovarian follicles, which are slowly depleted during their reproductive years until reproductive failure (menopause) occurs. The rate of loss of primordial follicles is determined by genetic and environmental influences, but certain toxic exposures can accelerate this process.
Connie J, Mark-Kappeler +2 more
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In vitro folliculogenesis of rat preantral follicles.
Endocrinology, 1995The impact of various gonadotropic hormones on the growth and development of secondary follicles from primordial and primary follicles obtained by enzymatic dissociation of the ovaries of immature 14-day-old rats was studied in vitro. The substratum-adherent culture technique developed for studying folliculogenesis in the current study permitted direct
L, Cain, S, Chatterjee, T J, Collins
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In vitro development of caprine ovarian preantral follicles
Theriogenology, 2000The in vitro growth and developmental pattern of caprine preantral follicles cultured in agar gel was observed. Preantral follicles 50 to 150 microm in diameter were isolated from prepuberal goat ovaries by treatment with collagenase and DNase. The isolated preantral follicles were cultured in agar gel for up to 14 days.
Z, Huanmin, Z, Yong
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In vitro development of sheep preantral follicles.
Biology of reproduction, 1999Preantral ovarian follicles isolated from prepubertal sheep ovaries were individually cultured for 6 days in the presence of increasing doses of FSH (ranging from 0.01 to 1 microg/ml) and under two different oxygen concentrations, 20% and 5% O2. Follicle development was evaluated on the basis of antral cavity formation as well as the presence of ...
CECCONI, Sandra +3 more
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Role of VEGF in pig preantral follicles
Veterinary Research Communications, 2008Role of VEGF in pig preantral follicles A. Martelli & V. Russo & A. Mauro & D. Nardinocchi & C. Rinaldi & N. Bernabo & P. Berardinelli & M.
MARTELLI, Alessandra +7 more
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Cryopreservation and in vitro culture of caprine preantral follicles
Reproduction, Fertility and Development, 2011Preantral follicles (PFs) form a far larger oocyte reservoir (∼90% of the follicular population) than antral follicles. Several laboratories have focussed efforts on cryopreservation and in vitro culture (IVC) of PFs to obtain large numbers of fertilisable oocytes.
J R, Figueiredo +3 more
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Isolation and long-term culture of human preantral follicles
Fertility and Sterility, 1993To isolate intact preantral follicles from the human ovary for in vitro studies.Premenopausal human ovary was digested by a mixture of collagenase and deoxyribonuclease (DNase) for 1 hour at 37 degrees C and for 36 hour at 4 degrees C. Intact preantral follicles at classes 1 and 2 were isolated and cultured for up to 120 hours in a serum-free Dulbecco ...
S K, Roy, B J, Treacy
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