Results 141 to 150 of about 5,100 (172)
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In vitro culture of buffalo (Bubalus bubalis) preantral follicles
Theriogenology, 2002Growth of buffalo preantral follicles in culture was studied to investigate the effect of size of preantral follicles, individual or group culture, long-term culture of preantral follicles for (40 days), addition of human follicle stimulating hormone (FSH), insulin-transferrin-selenium (ITS), growth factors (epidermal growth factor (EGF), fibroblast ...
P S P, Gupta +3 more
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Isolation and long-term culture of human preantral follicles
Fertility and Sterility, 1993To isolate intact preantral follicles from the human ovary for in vitro studies.Premenopausal human ovary was digested by a mixture of collagenase and deoxyribonuclease (DNase) for 1 hour at 37 degrees C and for 36 hour at 4 degrees C. Intact preantral follicles at classes 1 and 2 were isolated and cultured for up to 120 hours in a serum-free Dulbecco ...
S K, Roy, B J, Treacy
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Methods for Equine Preantral Follicles Isolation: Quantitative Aspects
Reproduction in Domestic Animals, 2013ContentsThe aim of this study was to test the use of mechanical and mechanical‐enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase).
E C R, Leonel +5 more
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In vitro Growth of Sheep Preantral Follicles: Which Perspectives?
Veterinary Research Communications, 2003G. Capacchietti1, S. Cecconi2, E. Colantoni2, V. Russo1, P. Berardinelli1, B. Barboni1 and M. Mattioli1 1Dip. Scienze Biomediche Comparate, Universita degli Studi di T eramo; 2Dip. Scienze e T ecnologie Biomediche,Universita degli Studi di L ’Aquila *Correspondence: Dip. Scienze Biomediche Comparate, sezione Fisiologia, Facolta di Medicina Veterinaria,
Cecconi S +6 more
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Morphogenetic reaggregation and luteinization of mouse preantral follicle cells
American Journal of Anatomy, 1979AbstractSmall (60‐90 μm) and large (100‐130 μm) preantral follicles were isolated from adult mouse ovaries by a collagenase‐dissection technique. These follicles were composed of resting oocytes surrounded either by granulosa cells, only, or by granulosa and undifferentiated theca cells.
S V, Nicosia, R, Tojo
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Theriogenology, 2015
Oocytes from preantral follicles could be an alternative for in vitro maturation because most follicles are at the preantral stage. There are few studies that have sought to estimate the number of preantral follicles in bitches. Therefore, the aims of this study were to estimate the population of preantral follicles in the ovaries of small- and medium ...
N T, Lunardon +5 more
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Oocytes from preantral follicles could be an alternative for in vitro maturation because most follicles are at the preantral stage. There are few studies that have sought to estimate the number of preantral follicles in bitches. Therefore, the aims of this study were to estimate the population of preantral follicles in the ovaries of small- and medium ...
N T, Lunardon +5 more
openaire +2 more sources
Zygote, 2007
SummaryThe aim of this study was to establish a basic manipulation protocol of preantral follicles for deriving developmentally competent oocytes. Primary, early and late secondary follicles retrieved from the ovaries of 14-day-old F1 (C57BL/6 × DBA2) female mice mechanically or enzymatically were cultured singly and in vitro growth of the follicles ...
S T, Lee +4 more
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SummaryThe aim of this study was to establish a basic manipulation protocol of preantral follicles for deriving developmentally competent oocytes. Primary, early and late secondary follicles retrieved from the ovaries of 14-day-old F1 (C57BL/6 × DBA2) female mice mechanically or enzymatically were cultured singly and in vitro growth of the follicles ...
S T, Lee +4 more
openaire +2 more sources
In vitro growth of preantral follicles in cats
EXP. 1 The objective was to determine effects of thyroxin (T4)and activinA on in vitro growth and morphology of preantral feline ovarian follicles. Preantral follicles (86.3± 18.7 µm) were isolated from fresh ovaries of domestic cats. Healthy follicles were cultured individually for 14 d in 20-µL microdrops of basic culture medium supplemented with ...openaire +1 more source
Isolation of preantral follicles from buffalo ovaries
Veterinary Record, 2001P S, Gupta +3 more
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