Results 161 to 170 of about 6,106 (203)

In vitro culture of buffalo (Bubalus bubalis) preantral follicles

Theriogenology, 2002
Growth of buffalo preantral follicles in culture was studied to investigate the effect of size of preantral follicles, individual or group culture, long-term culture of preantral follicles for (40 days), addition of human follicle stimulating hormone (FSH), insulin-transferrin-selenium (ITS), growth factors (epidermal growth factor (EGF), fibroblast ...
P S P Gupta, S Nandi, B M Ravindranatha
exaly   +3 more sources

The early stages of follicular development: activation of primordial follicles and growth of preantral follicles

Animal Reproduction Science, 2003
Although enormous progress has been made in understanding the events and regulation of the later stages of ovarian follicular development, the early stages of development, to a large extent and particularly in large mammals, remain a mystery. Mechanisms that regulate the initiation of follicular growth (follicle activation) and the ensuing growth and ...
J E Fortune
exaly   +3 more sources

Effect of sectioning on the number of isolated ovine preantral follicles

Small Ruminant Research, 2000
The aim of the present study was to test the effect of the interval of serial sections in the tissue chopper on the number of isolated ovine preantral follicles. Best results were obtained when the ovarian fragments were cut in the tissue chopper at interval of 87.5µm (1592 preantral follicles per treatment).
, Amorim, , Rodrigues, , Lucci, , Figueiredo, , Gonçalves   +4 more
openaire   +3 more sources

Preservation of oocyte and granulosa cell morphology in bovine preantral follicles cultured in vitro

Theriogenology, 1994
peer reviewedDescribed in the present paper is a culture system that preserves oocyte and granulosa cell morphology in bovine preantral follicles during 5 d in vitro.
J R Figueiredo, S C J Hulshof, R Van Den Hurk   +2 more
exaly   +2 more sources

Quantitative and qualitative analysis of the effectiveness of a mechanical method for the isolation of preantral follicles from ovine ovaries

Theriogenology, 2000
The preantral follicles are the major source of oocytes and its utilization has been investigated as an important tool to store large numbers of female gametes for further utilization in reproductive programs.
Christiani A Amorim, Carolina M Lucci, J R Figueiredo   +2 more
exaly   +2 more sources

In vitro culture of bovine preantral follicles

Animal Reproduction Science, 2000
Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM ...
S, Saha, M, Shimizu, M, Geshi, Y, Izaike
openaire   +2 more sources

Preantral follicle culture and oocyte quality

Reproduction, Fertility and Development, 2017
The formation of high-quality oocytes depends on complex stage-specific interactions between the germ cell and the somatic compartment involving endocrine, paracrine, and autocrine regulation. Cooperativity in bidirectional signalling and metabolism in response to factors in the microenvironment drive growth, proliferation, cell cycle regulation ...
Heiligentag, Martyna, Eichenlaub-Ritter, Ursula   +1 more
openaire   +2 more sources

In vitro culture methods of preantral follicles

Theriogenology, 2016
Folliculogenesis is a highly regulated, dynamic process requiring various autocrine, paracrine, and endocrine factors that need to be reproduced in vitro. The mammalian ovary consists of a finite number of follicles at birth, with the majority in a quiescent state, awaiting recruitment.
Lisa J, Green, Ariella, Shikanov
openaire   +2 more sources

Regulators of Ovarian Preantral Follicle Development

Seminars in Reproductive Medicine, 2015
Preantral follicle development has become an increasingly recognized area of study in the last two decades. Factors that regulate the growth survival and differentiation of these small, yet complex structures have been identified. The field of fertility preservation and a need for increased numbers of mature oocytes for stem cell research revealed how ...
Elizabeth A, McGee, Renju S, Raj
openaire   +2 more sources

Cryopreservation and in vitro culture of caprine preantral follicles

Reproduction, Fertility and Development, 2011
Preantral follicles (PFs) form a far larger oocyte reservoir (∼90% of the follicular population) than antral follicles. Several laboratories have focussed efforts on cryopreservation and in vitro culture (IVC) of PFs to obtain large numbers of fertilisable oocytes.
J R, Figueiredo, A P R, Rodrigues, J R V, Silva, R R, Santos   +3 more
openaire   +2 more sources