Results 71 to 80 of about 6,106 (203)
Sphingosine 1-phosphate promotes activation of aprine preantral follicle in vitro
This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro.
J.E. Nóbrega Jr. +10 more
doaj +1 more source
Zinc Deficiency Disrupts Germ Cell Nest Breakdown During In Vitro Ovary Culture
ABSTRACT In mammals, the size of the non‐renewable primordial follicle pool is established before or soon after birth. Primordial follicles, each composed of a single oocyte surrounded by somatic cells, are the only source of gametes during the entire reproductive lifespan of the female.
James M. Hester +3 more
wiley +1 more source
Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the ...
Reza Asadzadeh +5 more
doaj
TFAM‐Mediated mtDNA Replication is Essential for Developmental Competence of In Vitro Grown Oocytes
ABSTRACT Purpose Mitochondria are essential for oocyte maturation and early embryonic development, supplying ATP and maintaining mitochondrial DNA (mtDNA) integrity. During oogenesis, mtDNA undergoes dramatic amplification, but the mechanisms and functional significance of this process remain unclear. The purpose of this study was to elucidate the role
Son Quang Do +3 more
wiley +1 more source
Background Storage conditions during transportation of explanted ovaries are a critical step in setting up fertility preservation protocols in both animal and human fields.
Anna Rita Piras +10 more
doaj +1 more source
Methods for Equine Preantral Follicles Isolation: Quantitative Aspects
ContentsThe aim of this study was to test the use of mechanical and mechanical‐enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase).
Rivas Leonel, Ellen +5 more
openaire +3 more sources
Thyroid autoimmunity may exert a subtle influence on ovarian reserve through complex endocrine and immune mechanisms, with age‐specific variation. Although AMH alterations are observed, their clinical relevance for primary ovarian insufficiency, fertility, and treatment outcomes of assisted reproduction remains unclear, highlighting the need for ...
Akira Iwase +9 more
wiley +1 more source
The Role of Anethole in Reproductive Physiology and In Vitro Biotechnologies—A Review
ABSTRACT In vitro reproductive biotechnologies show promise for fertility preservation but still face challenges, including oxidative stress from high oxygen tension, which impairs cell viability and development. Antioxidants have been widely explored to attenuate oxidative stress during culture.
André Luiz da Conceição‐Santos +1 more
wiley +1 more source
Study question: Considering the early pool of gametes potentially able to generate offspring, what actions should be taken to optimize ivF thus increasing fertilizable oocytes availability?
Maurizio Monaci +6 more
core
Development of antral follicles after xenografting of isolated small human preantral follicles
Small human pre-antral follicles can be enzymatically isolated from the surrounding stroma, and are able to survive after 7 days of xenografting. The aim of the present study was to assess the developmental capacity of enzymatically isolated human follicles after long-term xenografting to severe combined immunodeficient (SCID) mice.
Marie-Madeleine, Dolmans +6 more
openaire +2 more sources

