Results 131 to 140 of about 2,166,973 (278)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Validation of predictive equations for resting energy expenditure in children and adolescents with different body mass indexes. [PDF]

Nutr J, 2023
Acar-Tek N, Ağagündüz D, Şahin TÖ, Baygut H, Uzunlar EA, Zakkour HK, Karaçallı A.   +6 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Development and validation of new predictive equations for resting energy expenditure in physically active boys. [PDF]

Sci Rep, 2023
Łuszczki E, Jagielski P, Bartosiewicz A, Dereń K, Matłosz P, Kuchciak M, Oleksy Ł, Stolarczyk A, Mazur A.   +8 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Energy expenditure and the accuracy of predictive equations in myotonic dystrophy type 1. [PDF]

J Neuromuscul Dis
Aarts WJA, van Veen BPAP, Fuchs CJ, Côté C, Hover L, van Loon LJC, Keszthelyi D, Mul K, Faber CG, Joosten IBT.   +9 more
europepmc   +1 more source

Resting energy expenditure in adolescents with Down syndrome: a comparison of commonly used predictive equations. [PDF]

J Intellect Disabil Res, 2023
Helsel BC, Shook RP, Forseth B, Dreyer Gillette ML, Polfuss M, Miller B, Posson P, Steele R, Thyfault JP, Ptomey LT.   +9 more
europepmc   +1 more source

Comparative predictive modeling of pediatric spirometry reference equations in Jordanian children: Complex versus simple models

Walid Al-Qerem, Anan Jarab, Judith Eberhardt, Khalda Smairan, Yousef Mimi, Maher R. Khdour   +5 more
openalex   +1 more source

Comparative predictive performance of different eGFR equations for 1-year major adverse cardiovascular events after percutaneous coronary intervention in patients with acute coronary syndrome [PDF]

Yicheng Wang, Liu Fangming, Ruoxi Qi, Xu Wang, Jiajie Mei, Zhenzhu Liu, Lijiao Zhang, Wenli Xie, Ming Yu, Xiaodong Zhang, Hongyan Wang, Zhaohong Geng   +11 more
openalex   +1 more source