Evaluation of Functional T-Cell Assays That Predict Causal Allergens in Eosinophilic Esophagitis
DiagnosticsBackground: Eosinophilic esophagitis (EoE) is a chronic, food antigen-driven disease of the esophagus that causes considerable morbidity. Elimination of allergenic foods from a patient’s diet is a highly effective treatment.
Julianna Dilollo +10 more
doaj +5 more sources
A performance evaluation of commercial fibrinogen reference preparations and assays for Clauss and PT-derived fibrinogen [PDF]
, 2002 The wide availability of fibrinogen estimations based on the prothrombin time (PT-Fg) has caused concern about the variability and clinical utility of fibrinogen assays.
Lowe, G.D.O. +19 more
core +9 more sources
Functional assays to screen and select monoclonal antibodies that target Yersinia pestis
Human Vaccines & Immunotherapeutics, 2023 Yersinia pestis is a gram-negative bacterium that causes plague in animals and humans. Depending on the route of disease transmission, the bacterium can cause an acute, often fatal disease that has a narrow window for treatment with antibiotics ...
Sergei S. Biryukov +14 more
doaj +2 more sources
Predictive chemoproteomics and functional validation reveal Coeae6g-mediated insecticide cross-resistance in the malaria vector Anopheles gambiae [PDF]
Nature CommunicationsSustaining malaria control in Africa is imperilled by the rapid evolution of insecticide resistance in the major vector Anopheles gambiae. Although current genomic and transcriptomic approaches map known resistance alleles, they often lack predictive ...
Sofia Balaska +8 more
doaj +2 more sources
Benchmarking deep learning splice prediction tools using functional splice assays [PDF]
Human Mutation, 2020 Hereditary disorders are frequently caused by genetic variants that affect pre-mRNA splicing. Whilst genetic variants in the canonical splice motifs are almost always disrupting splicing, the pathogenicity of variants in the non-canonical splice sites (NCSS) and deep intronic (DI) regions are difficult to predict.
Tabea V. Riepe +4 more
openaire +4 more sources
Development of a rapid functional assay that predicts GLUT1 disease severity [PDF]
Neurology Genetics, 2018 To examine the genotype to phenotype connection in glucose transporter type 1 (GLUT1) deficiency and whether a simple functional assay can predict disease outcome from genetic sequence alone.GLUT1 deficiency, due to mutations in SLC2A1, causes a wide range of epilepsies. One possible mechanism for this is variable impact of mutations on GLUT1 function.
Zaman, SM +15 more
openaire +3 more sources
Compound Functional Prediction Using Multiple Unrelated Morphological Profiling Assays [PDF]
SLAS Technology, 2018 Phenotypic cell-based assays have proven to be efficient at discovering first-in-class therapeutic drugs mainly because they allow for scanning a wide spectrum of possible targets at once. However, despite compelling methodological advances, posterior identification of a compound's mechanism of action (MOA) has remained difficult and highly refractory ...
France, Rose +5 more
openaire +2 more sources
Andrology: Two functional assays of sperm responsiveness to progesterone and their predictive values in in-vitro fertilization [PDF]
Human Reproduction, 1996 We have recently reported, in a small cohort of subjects, that acrosome reaction (AR) and intracellular free calcium ([Ca2+]i) increase in response to progesterone were significantly correlated with in-vitro fertilization (IVF) rate. In the present study we extended these results to 90 subjects undergoing IVF.
KRAUSZ, CSILLA GABRIELLA +8 more
openaire +3 more sources
Contribution of bioinformatics predictions and functional splicing assays to the interpretation of unclassified variants of the BRCA genes [PDF]
European Journal of Human Genetics, 2011 A large fraction of sequence variants of unknown significance (VUS) of the breast and ovarian cancer susceptibility genes BRCA1 and BRCA2 may induce splicing defects. We analyzed 53 VUSs of BRCA1 or BRCA2, detected in consecutive molecular screenings, by using five splicing prediction programs, and we classified them into two groups according to the ...
Théry, Jean Christophe +14 more
openaire +4 more sources