Results 81 to 90 of about 874,956 (269)

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Machine learning-driven prediction model for cuproptosis-related genes in spinal cord injury: construction and experimental validation

Frontiers in Neurology
IntroductionSpinal cord injury (SCI) severely affects the central nervous system. Copper homeostasis is closely related to mitochondrial regulation, and cuproptosis is a novel form of cell death associated with mitochondrial metabolism.
Yimin Zhou, Xin Li, Zixiu Wang, Liqi Ng, Rong He, Chaozong Liu, Gang Liu, Xiao Fan, Xiaohong Mu, Yu Zhou, Yu Zhou   +10 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

On the Relevance and Limitations of Predictive Models in Clinical Psychology

Mediterranean Journal of Clinical Psychology
Following a relevant contribution promoted by the American Psychological Association (Hanson, 2022), prediction statistics in Clinical Psychology represent a series of practices useful to predict the effect of an independent variable on possible outcomes.
Emanuele Maria Merlo
doaj   +1 more source

Locally induced predictive models

2011 IEEE International Conference on Systems, Man, and Cybernetics, 2011
Most predictive modeling techniques utilize all available data to build global models. This is despite the wellknown fact that for many problems, the targeted relationship varies greatly over the input space, thus suggesting that localized models may improve predictive performance.
Ulf Johansson, Tuve Löfström, Cecilia Sönströd   +2 more
openaire   +4 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Risk Assessment Profiles for Caregiver Burden in Family Caregivers of Persons Living with Alzheimer’s Disease: An Exploratory Study with Machine Learning

European Journal of Investigation in Health, Psychology and Education
Alzheimer’s disease (AD) places a profound global challenge, driven by its escalating prevalence and the multifaceted strain it places on individuals, families, and societies. Family caregivers (FCs), who are pivotal in supporting family members with AD,
Laura Brito, Beatriz Cepa, Cláudia Brito, Ângela Leite, M. Graça Pereira   +4 more
doaj   +1 more source

Locations of Deer–Vehicle Collisions are Unrelated to Traffic Volume or Posted Speed Limit

Human-Wildlife Interactions, 2017
Consensus is lacking regarding the influence of vehicle speed and traffic volume on deer–vehicle collision (DVC) rates. Yet, annual average daily traffic fl ow (AADT) and posted speed limit (PSL) typically are used to measure these variables.
John A. Bissonette, Christine A. Kassar
doaj   +1 more source

Models for Understanding Versus Models for Prediction [PDF]

, 2008
According to a standard point of view, statistical modelling consists in establishing a parsimonious representation of a random phenomenon, generally based upon the knowledge of an expert of the application field: the aim of a model is to provide a better understanding of data and of the underlying mechanism which have produced it.
openaire   +3 more sources