Results 31 to 40 of about 1,405,774 (308)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Effect of Drive Side Pressure Angle and Addendum on Mesh Stiffness of the Gears with Low and High Contact Ratios

Applied Sciences
Gears are one of the most important machine elements widely used to transmit motion and power in various machines. The gear tooth stiffness has a significant impact on the load distribution, vibration characteristics, and overall efficiency of gear ...
Nurullah Baris Sandikci, Ozdes Cermik, Oguz Dogan   +2 more
doaj   +1 more source

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Investigating the Coupling Effect of High Pressure and Hot Air on External Friction Angle Based on Resistance Reduction Tests on Subsoiling Tillage Tools for Sandy Clay Loam

Agronomy, 2022
Sandy clay loam has the characteristics of both sand and clay. Because of these characteristics, both frictional resistance and adhesive resistance occur between the soil and tillage tool.
Kuan Qin, Yongzheng Zhang, Zhougao Shen, Chengmao Cao, Zhengmin Wu, Jun Ge, Liangfei Fang, Haijun Bi   +7 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Pengaruh Woven Angle dan Proses Penekanan terhadap Tegangan Tarik pada Natural Fiber Laminate Composite

Rekayasa Mesin, 2019
The aim of this study is to investigate the effects of pressure and woven angle on the tensile strength of natural fiber laminate composite. Pandanus tectorius and polymethyl methacrylate (PMMA) was used as natural fiber and matrix, respectively.
Khairul Anam, Anindito Purnowidodo, Sofyan Arif Setyabudi   +2 more
doaj   +1 more source

Analytical and Numerical Contact Stress Analysis of Spur Gears

Science, Engineering and Technology, 2023
The damage that occurs on the teeth flank of cylindrical gears is a complex phenomenon and depends on many factors. The most common cause for these damages is the high contact stress of the meshing gears.
Blagoja Nestorovski, Petar Simonovski
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source