Results 91 to 100 of about 373,369 (294)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

An experimental study of surface pressure fluctuations in a separating turbulent boundary layer [PDF]

Measurements of streamwise velocity fluctuation and surface pressure fluctuation spectra and wavespeeds are reported for a well-documented separating turbulent boundary layer. Because a portion of the acoustic pressure fluctuations is the same across the
Ghodbane, M., Mcgrath, B. E., Simpson, Roger L.   +2 more
core   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Numerical Calculation of the Influence of Working Conditions and Rotational Speed on Pressure Pulsation of a Self‐Priming Pump

Energy Science & Engineering
To accurately determine the pressure fluctuation characteristics of a specific self‐priming pump, a full three‐dimensional computational model was established, including the S‐tube, impeller domain, volute domain, gas‐liquid separation chamber, front and
Hai‐Bing Jiang, Shao‐Han Zheng, Yu‐Liang Zhang, Liang‐Huai Tong, Jin‐Fu Li, He‐Chao Guo   +5 more
doaj   +1 more source

Pressure fluctuation–vortex interaction in an ultra-low specific-speed centrifugal pump

Journal of Low Frequency Noise, Vibration and Active Control, 2019
To provide a comprehensive understanding of the pressure fluctuation–vortex interaction in non-cavitation and cavitation flow, in this article, the unsteady flow in an ultra-low specific-speed centrifugal pump was investigated by numerical simulation ...
Cong Wang, Yongxue Zhang, Zhiwei Li, Ao Xu, Chang Xu, Zhicheng Shi   +5 more
doaj   +1 more source

Wind tunnel measurements of surface pressure fluctuations at Mach numbers of 1.6, 2.0, and 2.5 using 12 different transducers [PDF]

The turbulent boundary layer on the wall of a 9 by 7 foot wind tunnel was measured with 12 different transducers at Mach numbers of 1.6, 2.0, and 2.5. The results indicated that the wall surface-pressure-fluctuation field was more homogeneous at a Mach ...
Dods, J. B., Jr., Lewis, T. L.
core   +1 more source

Equation of state of charged colloidal suspensions and its dependence on the thermodynamic route

, 2012
The thermodynamic properties of highly charged colloidal suspensions in contact with a salt reservoir are investigated in the framework of the Renormalized Jellium Model (RJM).
Alexandre P. dos Santos, Allen M. P., Denton A. R., Derjaguin B. V., Hansen J.-P, Thiago E. Colla, Verwey E. J., Yan Levin   +7 more
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source