Results 241 to 250 of about 93,364 (279)

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Cell, 2021
While prime editing enables precise sequence changes in DNA, cellular determinants of prime editing remain poorly understood. Using pooled CRISPRi screens, we discovered that DNA mismatch repair (MMR) impedes prime editing and promotes undesired indel ...
Peter J Chen, Jeffrey A Hussmann, Friederike Knipping   +2 more
exaly   +2 more sources

Prediction of efficiencies for diverse prime editing systems in multiple cell types

Cell, 2023
Applications of prime editing are often limited due to insufficient efficiencies, and it can require substantial time and resources to determine the most efficient pegRNAs and prime editors (PEs) to generate a desired edit under various experimental ...
Goosang Yu, Hui Kwon Kim, Jihye Park
exaly   +3 more sources

Improved prime editing allows for routine predictable gene editing in Physcomitrium patens [PDF]

Journal of Experimental Botany, 2023
Efficient and precise gene editing is the gold standard of any reverse genetic study. The recently developed prime editing approach, a modified CRISPR/Cas9 [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein] editing ...
Pierre-François Perroud, Anouchka Guyon-Debast, Josep M Casacuberta   +2 more
exaly   +2 more sources

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Editing to Prime Graphs

Bulletin of the Malaysian Mathematical Sciences Society, 2021
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Houmem Belkhechine, Cherifa Ben Salha
openaire   +1 more source

Prime Editing: A New Way for Genome Editing

Trends in Cell Biology, 2020
Precise and efficient use of genome editing tools are hampered by the introduction of DNA double-strand breaks, donor DNA templates, or homology-directed repair. A recent study expands the genome editing toolbox with the introduction of prime editing, which overcomes previous challenges and introduces insertions, deletions, and all putative 12 types of
Hensel, Goetz, Marzec, Marek, Braszewska-Zalewska, Agnieszka   +2 more
openaire   +3 more sources

Prime editing progress

Nature Methods, 2021
Researchers work towards improving the efficiency and specificity of prime editors, as well as their delivery system in plants and animals.
openaire   +2 more sources

Prime editing in mice with an engineered pegRNA

Vascular Pharmacology, 2023
CRISPR editing involves double-strand breaks in DNA with attending insertions/deletions (indels) that may result in embryonic lethality in mice. The prime editing (PE) platform uses a prime editing guide RNA (pegRNA) and a Cas9 nickase fused to a modified reverse transcriptase to precisely introduce nucleotide substitutions or small indels without the ...
Amr R, Salem, W Bart, Bryant, Jaser, Doja, Susan H, Griffin, Xiaofan, Shi, Weihong, Han, Yunchao, Su, Alexander D, Verin, Joseph M, Miano   +8 more
openaire   +2 more sources

Optimization of Prime Editing in Rice, Peanut, Chickpea, and Cowpea Protoplasts by Restoration of GFP Activity

International Journal of Molecular Sciences, 2022
Precise editing of the plant genome has long been desired for functional genomic research and crop breeding. Prime editing is a newly developed precise editing technology based on CRISPR-Cas9, which uses an engineered reverse transcriptase (RT), a ...
Sudip Biswas, Samra Irum, Michael J Thomson   +2 more
exaly   +2 more sources

Introduction of mutations in plants with prime editing

Methods, 2021
The emergence of a clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has had a revolutionary impact on plant biology. However, this system and further developed base editing are limited by their inherent imperfection.
Lijun, Hao, Xiangdong, Pu, Jingyuan, Song   +2 more
openaire   +2 more sources

Current knowledge of base editing and prime editing

Molekulârnaâ biologiâ
Modern genetic engineering technologies, such as base editing (BE) and prime editing (PE), have proven to be effective and reliable genome editing tools that do not require the introduction of double-strand breaks in DNA and the presence of donor templates.
O A, Averina, S A, Kuznetsova, O A, Permyakov, P V, Sergiev   +3 more
openaire   +2 more sources