Results 251 to 260 of about 93,364 (279)

Optimizing Prime Editing in Zebrafish

The CRISPR Journal
Prime editing is a clustered regularly interspaced short palindromic repeats-based approach that enables the introduction of precise genetic modifications, including missense mutations, making it valuable for generating disease models. The comparative performance of novel prime editor (PE) variants in zebrafish remains largely unexplored.
Rehman Basharat, Gina Rizzo, Josiah D. Zoodsma, Lonnie P. Wollmuth, Howard I. Sirotkin   +4 more
openaire   +2 more sources

Prime genome editing in rice and wheat

Nature Biotechnology, 2020
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization.
Qiupeng Lin, Yuan Zong, Chenxiao Xue, Shengxing Wang, Shuai Jin, Zixu Zhu, Yanpeng Wang, Andrew V. Anzalone, Aditya Raguram, Jordan L. Doman, David R. Liu, Caixia Gao   +11 more
openaire   +2 more sources

Prime Editing in Mammals: The Next Generation of Precision Genome Editing

The CRISPR Journal, 2022
The recently established prime editor (PE) system is regarded as next-generation gene-editing technology. This methodology can install any base-to-base change as well as insertions and deletions without the requirement for double-stranded break formation or donor DNA templates; thus, it offers more targeting flexibility and greater editing precision ...
Dawei Wang, Xiude Fan, Mengzhu Li, Tianbo Liu, Peng Lu, Guangxin Wang, Yuan Li, JunMing Han, JiaJun Zhao   +8 more
openaire   +2 more sources

The Advancement of Prime Editing Technology

ChemBioChem
The advent of CRISPR/Cas genome editing has spurred major breakthroughs across life sciences, offering vast potential across numerous research and application fields. Among the expanding toolkit of CRISPR/Cas‐derived methods, prime editing (PE) stands out for its versatility and specificity, enabling precise point mutations and small insertions or ...
Lu Zhang, Dongdong Zhao, Zhandong Wei, Xiao Zhu, Taixin Sha, Wenzhu Tang, Changhao Bi, Xueli Zhang   +7 more
openaire   +2 more sources

Prime editing: The state-of-the-art of genome editing

Meta Gene, 2020
Abstract Several diseases are the consequence of genetic alterations in normal DNA sequences. Solving the problem of these alterations can be possible through genome editing which exactly means replacing the wrong sequence with the correct ones. One of the common tools for this repair is clustered regularly interspaced short palindromic repeats ...
Fatemeh Khatami, Maryam Aghaii, Seyed Mohammad Kazem Aghamir   +2 more
openaire   +1 more source

Engineering CjCas9 for Efficient Base Editing and Prime Editing

The CRISPR Journal
The CRISPR-Cas9 system has been applied for clinical applications of gene therapy. Most CRISPR-based gene therapies are derived from Streptococcus pyogenes Cas9, which is challenging to package into a single adeno-associated virus vector and limits its clinical applications.
Siyuan Liu, Yingdi Zhao, Qiqin Mo, Yadong Sun, Hanhui Ma   +4 more
openaire   +2 more sources

Plant prime editing goes prime

Nature Plants, 2021
Simon Sretenovic, Yiping Qi
openaire   +2 more sources

Prime editing in bacteria with BacPE

Programmable genome editing technologies have revolutionized the ability of researchers to alter the genomes of microorganisms in a straightforward and efficient manner, significantly advancing the field of microbiology. To date, several CRISPR-Cas-based genome-editing systems have been developed for use in E. coli, including CRISPR/Cas9, base editing,
Hongyuan, Zhang, Quanjiang, Ji
openaire   +2 more sources

Extended pegRNAs enhance the editing capability of Prime editing

Trends in Biotechnology
Genome editing is highly valuable in biomedical research. Despite their versatility, current Prime editing (PE) techniques are limited to short sequence alterations [up to ~44 base pairs (bp)], and exhibit inconsistent or low efficiency across genomic loci, particularly when faced with poly-T sequences.
Kezhang He, Qiaomei Xue, Wei Zhou, Pengqi Wang, Xiaodan Hu, Tongtong Lin, Nan Chen, Bowen Wang, Tianhua Ma, Sheng Ding   +9 more
openaire   +2 more sources

Perfecting prime editing: achieving precise edits in dicots

Trends in Plant Science
Prime editing (PE), a precise CRISPR-based method, has worked well in some plants but faces challenges in dicots. Vu and colleagues developed new PE tools that greatly improve PE efficiency in dicots, enabling accurate, heritable genome edits. This advance marks a breakthrough that could revolutionize crop improvement and plant biotechnology.
Niaz Ahmad, Muhammad Jawad Akbar Awan, Imran Amin, Shahid Mansoor   +3 more
openaire   +2 more sources