Results 261 to 270 of about 93,364 (279)

Prime editing in plants: prospects and challenges

Journal of Experimental Botany
Abstract Prime editors are reverse transcriptase (RT)-based genome-editing tools that utilize double-strand break (DSB)-free mechanisms to decrease off-target editing in genomes and enhance the efficiency of targeted insertions. The multiple prime editors that have been developed within a short span of time are a testament to the ...
Sanskriti Vats, Jitesh Kumar, Humira Sonah, Feng Zhang, Rupesh Deshmukh   +4 more
openaire   +2 more sources

Prime Editing of Mouse Primary Neurons

Prime editing is a hybrid genome editing technology that introduces small edits on the genome with high precision. It combines nickase Cas9 with reverse transcriptase to prime and synthesizes edited DNA from RNA, reducing unintended insertions and deletions on the genome. This protocol describes the design of prime editing guide RNAs (pegRNAs), cloning
Colin D, Robertson, Ryan R, Richardson, Marilyn, Steyert, Corinne A, Martin, Corey, Flynn, Alexandros, Poulopoulos   +5 more
openaire   +2 more sources

Prime-Editing Methods and pegRNA Design Programs

Molecular Biology
It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed. BE is performed via the formation of single-strand breaks by the mutant
E V, Mikhaylova, B R, Kuluev, G A, Gerashchenkov, D A, Chemeris, R R, Garafutdinov, A R, Kuluev, An K, Baymiev, Al K, Baymiev, A V, Chemeris   +8 more
openaire   +2 more sources

Bidirectional Prime Editing: Combining Precision with Versatility for Genome Editing

Cellular Reprogramming
Genome editing techniques have potential to revolutionize the field of life sciences. Several limitations associated with traditional gene editing techniques have been resolved with the development of prime editors that precisely edit the DNA without double-strand breaks (DSBs).
Mahmood S. Choudhery, Taqdees Arif, Ruhma Mahmood   +2 more
openaire   +2 more sources

The promise of in vivo HSC prime editing

Blood, 2023
Colin L. Sweeney, Suk See De Ravin
openaire   +2 more sources

Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease

Cell Stem Cell, 2021
Yeseul Kim, Sung-Ah Hong, Jihyeon Yu
exaly  

A truncated reverse transcriptase enhances prime editing by split AAV vectors

Molecular Therapy, 2022
Zongliang Gao, Huiqiang Cai, Søren R Paludan   +2 more
exaly  

Priming crops for heat stress with prime editing

Science China Life Sciences
Karla, Gasparini, Agustin, Zsögön
openaire   +2 more sources

Plant base editing and prime editing: The current status and future perspectives

Journal of Integrative Plant Biology, 2023
Jingying Li, Yubing He, Shaoya Li
exaly  

An optimized toolkit for prime editing

Nature Biotechnology
Ryan M. Marks, Ori Scott, Evgueni A. Ivakine, Ronald D. Cohn   +3 more
openaire   +2 more sources