Results 71 to 80 of about 93,364 (279)
WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing
Signal Transduction and Targeted Therapy, 2022 Large scale genomic aberrations including duplication, deletion, translocation, and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of cancer.
Rui Tao +11 more
doaj +1 more source
FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source
Expansion of the prime editing modality with Cas9 from Francisella novicida
Genome Biology, 2022 Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase.
Yeounsun Oh +16 more
doaj +1 more source
FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source
Lentiviral delivery of CRISPR Cas9 prime editing system for cystic fibrosis
The field of gene editing is rapidly developing, particularly with advancements in gene editor technologies. Prime editing has been a significant breakthrough in gene editing, allowing precise, targeted correction of all types of DNA mutation, including ...
Satyapertiwi, Dwiantari
core +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Peptide fusion improves prime editing efficiency
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, PepSEq, to measure how fusion of 12,000 85-amino acid peptides derived from human DNA repair-related proteins ...
Velimirovic, Minja
core +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
, 2021 The purpose of this research is to evaluate the potential of the CRISPR-derived class of prime editors for precise correction of the E342K mutation in Alpha-1 Antitrypsin Deficiency (A1AD) and to discover genetic dependencies of the prime editing ...
Lung, Genesis
core
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
, 2020 CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing.
Xulin Hong +5 more
core +1 more source