Results 81 to 90 of about 93,364 (279)
The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome
FEBS Letters, EarlyView.This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley +1 more source
Prime editing – an update on the field [PDF]
Gene Therapy, 2021 Janine Scholefield, Patrick T. Harrison
openaire +2 more sources
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
WES data R201H introcution in HEK cells using prime editing
, 2023 WES dataset of HEK cell pool transfected with prime editing to introduce the KCNQ2 R201H ...
Nina Dirkx (14854792)
core +1 more source
Robust and inducible genome editing via an all-in-one prime editor in human pluripotent stem cells
Nature CommunicationsPrime editing (PE) allows for precise genome editing in human pluripotent stem cells (hPSCs), such as introducing single nucleotide modifications, small insertions or deletions at a specific genomic locus. Here, we systematically compare a panel of prime
Youjun Wu +7 more
doaj +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing
, 2022 Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install precise genomic insertions using an SpCas9 nuclease-based prime editor ...
Mendoza-Garcia, P. +13 more
core +1 more source
FEBS Letters, EarlyView.Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara +3 more
wiley +1 more source
, 2023 Breast cancer is the most common cancer among women in Finland and worldwide, but, fortunately, the survival rate in Finland is over 90 %. Genome instability and mutations are one reason for the formation of cancer.
Nikku, Laura
core
Prime editing in zebrafish and Atlantic salmon : exploring a new CRISPR technique in non-mammalian species [PDF]
, 2022 The CRISPR-Cas system has enabled precise genome editing in a multitude of organisms, and gene knockout experiments can now be done with relative ease due to the simplicity of the system.
Bryhni, Jenny Heyerdahl
core