Results 91 to 100 of about 93,364 (279)
Additional file 1 of Expansion of the prime editing modality with Cas9 from Francisella novicida
, 2022 Additional file 1: Figure S1. Analysis of the cleavage point of FnCas9 and SpCas9. Figure S2. Shared target sequence and editing method by using SpCas9(H840A)-RT and FnCas9(H969A)-RT. Figure S3.
Kyung-Seob Lim (12405135) +16 more
core +1 more source
Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers
Molecular Oncology, EarlyView.This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel +6 more
wiley +1 more source
Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
, 2023 openLa distrofia muscolare di Duchenne (DMD) è una malattia genetica rara e mortale, causata dalla mancanza di distrofina. Le mutazioni nel gene DMD, localizzato nel cromosoma X, sono trasmesse in modo recessivo legato al sesso; in particolare, una ...
DE ANTONI, NOEMI
core
Molecular Oncology, EarlyView.Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano +6 more
wiley +1 more source
An investigation of prime editing kinetics [PDF]
DNA spielt eine grundlegende Rolle in lebenden Systemen, indem sie genetische Informationen speichert und reguliert. Neue Entwicklungen wie Prime Editing ermöglichen präzise Modifikationen der Basenpaare des Genoms und versprechen damit verbesserte ...
Schäffler, Nathalie
core
Deletion and replacement of long genomic sequences using prime editing
, 2021 Genomic insertions, duplications and insertion/deletions (indels), which account for ~14% of human pathogenic mutations, cannot be accurately or efficiently corrected by current gene editing methods, especially those that involve larger alterations (>100
Xue, Wen +3 more
core +1 more source
Molecular Oncology, EarlyView.Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau +36 more
wiley +1 more source
Predicting CRISPR-Cas9 Prime Editing Efficiency across Diverse Edits and Chromatin Contexts with Machine Learning [PDF]
Genome editing offers the unprecedented ability to precisely modify genetic material, signaling a new era in our approach to understanding life and treating genetic diseases.
Mathis, Nicolas
core +1 more source
Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer
Molecular Oncology, EarlyView.COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann +61 more
wiley +1 more source
Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer
Molecular Oncology, EarlyView.Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni +11 more
wiley +1 more source