Results 281 to 290 of about 5,438,748 (355)

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Global medium-term research priority setting for seasonal malaria chemoprevention: results from an eDelphi study. [PDF]

BMJ Glob Health
Richardson S, Steele A, Scott S, Ainsworth J, Eckert E, Tchouatieu AM, Sillah JS, Cuomo-Dannenburg G, Okereke E, Okeh DU, Tonks S, Ndiaye JL, Dentinger C, Rassi C, Baker K.   +14 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Research priorities for the most premature babies born <25 weeks' gestation: results of an international priority setting partnership. [PDF]

Arch Dis Child Fetal Neonatal Ed
Peart S, Ray O, Galletta L, Bates A, Boland RA, Davis PG, Gale C, Johnson S, Kinsella S, Knight M, Owen LS, Pallot L, Prentice TM, Santhanadass P, Stanbury K, Tingay D, Whitehead CL, Manley BJ, Roehr CC, Hardy P, Steering Group.   +20 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Priority setting in critical care: a multicriteria approach to ranking access to Intensive Care Unit beds. [PDF]

BMC Health Serv Res
Dos Santos AFA, Dos Santos LA, Frazão TDC, de Assis AG, de Oliveira MSC, da Costa Júnior JF, da S Ferro PAT, de Souza RP.   +7 more
europepmc   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Research priorities for faecal incontinence in adults: A James Lind Alliance priority setting partnership. [PDF]

Colorectal Dis
O'Connor A, Rose SA, Carrington EV, Clements A, Cornish JA, Drake MJ, Dunford LJ, Grainger J, Hallett D, Lough K, Marunda T, Sesay A, Vasant DH, Willson T, Dudding T.   +14 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source